Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   B7P25_RS22395 Genome accession   NZ_CP020723
Coordinates   4121429..4122175 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus thuringiensis strain BM-BT15426     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4116429..4127175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7P25_RS22365 (B7P25_22315) dnaG 4117197..4118993 (-) 1797 WP_000528216.1 DNA primase -
  B7P25_RS28990 (B7P25_22325) - 4119324..4119479 (-) 156 WP_001179661.1 hypothetical protein -
  B7P25_RS28755 (B7P25_22330) - 4119668..4119811 (-) 144 WP_153576741.1 hypothetical protein -
  B7P25_RS22385 (B7P25_22335) - 4119847..4120659 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  B7P25_RS22390 (B7P25_22340) - 4120689..4121321 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  B7P25_RS22395 (B7P25_22345) recO 4121429..4122175 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  B7P25_RS22400 (B7P25_22350) - 4122209..4122352 (-) 144 WP_000883924.1 YqzL family protein -
  B7P25_RS22405 (B7P25_22355) era 4122529..4123434 (-) 906 WP_001080241.1 GTPase Era -
  B7P25_RS22410 (B7P25_22360) - 4123427..4123825 (-) 399 WP_087932366.1 cytidine deaminase -
  B7P25_RS22415 (B7P25_22365) - 4123925..4124278 (-) 354 WP_001989858.1 diacylglycerol kinase family protein -
  B7P25_RS22420 (B7P25_22370) ybeY 4124275..4124745 (-) 471 WP_000054692.1 rRNA maturation RNase YbeY -
  B7P25_RS22425 (B7P25_22375) - 4124742..4126886 (-) 2145 WP_000946454.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=225559 B7P25_RS22395 WP_000487010.1 4121429..4122175(-) (recO) [Bacillus thuringiensis strain BM-BT15426]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=225559 B7P25_RS22395 WP_000487010.1 4121429..4122175(-) (recO) [Bacillus thuringiensis strain BM-BT15426]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTTTCAAG
AGAATTGGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGTCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGACACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGAGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTACCGTATACCAGTGGG
GGAAGCTGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGGCTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment