Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   B7P25_RS22115 Genome accession   NZ_CP020723
Coordinates   4068641..4069684 (-) Length   347 a.a.
NCBI ID   WP_001013211.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain BM-BT15426     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4063641..4074684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7P25_RS22070 (B7P25_22020) - 4064068..4064268 (-) 201 WP_000106081.1 YqzE family protein -
  B7P25_RS22075 (B7P25_22025) aroK 4064307..4064804 (-) 498 WP_000836631.1 shikimate kinase AroK -
  B7P25_RS22080 (B7P25_22030) - 4064925..4065575 (-) 651 WP_000183878.1 2OG-Fe(II) oxygenase -
  B7P25_RS22085 (B7P25_22035) comGG 4065751..4066122 (-) 372 WP_001231192.1 competence type IV pilus minor pilin ComGG -
  B7P25_RS22090 (B7P25_22040) comGF 4066119..4066589 (-) 471 WP_000923094.1 competence type IV pilus minor pilin ComGF -
  B7P25_RS22095 (B7P25_22045) comGE 4066559..4066861 (-) 303 WP_000829456.1 competence type IV pilus minor pilin ComGE -
  B7P25_RS22100 (B7P25_22050) comGD 4066854..4067309 (-) 456 WP_000810395.1 comG operon protein ComGD -
  B7P25_RS22105 (B7P25_22055) comGC 4067306..4067605 (-) 300 WP_001178696.1 comG operon protein ComGC -
  B7P25_RS22110 (B7P25_22060) comGB 4067617..4068648 (-) 1032 WP_000471911.1 comG operon protein ComGB -
  B7P25_RS22115 (B7P25_22065) comGA 4068641..4069684 (-) 1044 WP_001013211.1 competence protein ComGA Machinery gene
  B7P25_RS22120 (B7P25_22070) - 4069890..4070585 (+) 696 WP_000434117.1 helix-turn-helix transcriptional regulator -
  B7P25_RS22125 (B7P25_22075) - 4070711..4070953 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  B7P25_RS22130 (B7P25_22080) - 4071061..4072455 (+) 1395 WP_001094329.1 L-cystine transporter -
  B7P25_RS22135 (B7P25_22085) - 4072631..4073038 (-) 408 WP_000877905.1 hypothetical protein -
  B7P25_RS22140 (B7P25_22090) - 4073126..4073341 (-) 216 WP_001008320.1 DUF3912 family protein -
  B7P25_RS22145 (B7P25_22095) - 4073594..4073905 (+) 312 WP_001093243.1 hypothetical protein -
  B7P25_RS22150 (B7P25_22100) - 4073941..4074429 (-) 489 WP_000764252.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39318.80 Da        Isoelectric Point: 9.0775

>NTDB_id=225558 B7P25_RS22115 WP_001013211.1 4068641..4069684(-) (comGA) [Bacillus thuringiensis strain BM-BT15426]
MNGIESFANTILKEACRVQASDLHIVPRQKDVVVQLRVGKDLMTKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKILLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=225558 B7P25_RS22115 WP_001013211.1 4068641..4069684(-) (comGA) [Bacillus thuringiensis strain BM-BT15426]
ATGAATGGAATTGAAAGCTTTGCGAATACGATTTTGAAAGAAGCGTGCAGGGTGCAAGCGTCGGACTTACATATTGTACC
CCGGCAGAAGGATGTAGTGGTTCAACTGCGTGTAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAAGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGATACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Vibrio campbellii strain DS40M4

35.511

100

0.36

Multiple sequence alignment