Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B7P25_RS20860 Genome accession   NZ_CP020723
Coordinates   3868806..3869240 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus thuringiensis strain BM-BT15426     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3863806..3874240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7P25_RS20840 (B7P25_20790) - 3864569..3865126 (+) 558 WP_000062080.1 PadR family transcriptional regulator -
  B7P25_RS20850 (B7P25_20800) metE 3866055..3868343 (+) 2289 WP_087932319.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  B7P25_RS20855 (B7P25_20805) comJ 3868380..3868769 (-) 390 WP_000424033.1 competence protein ComJ -
  B7P25_RS20860 (B7P25_20810) nucA/comI 3868806..3869240 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  B7P25_RS20870 (B7P25_20820) - 3869477..3870226 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  B7P25_RS20875 (B7P25_20825) kinB 3870280..3871554 (-) 1275 WP_000420390.1 sporulation sensor histidine kinase KinB -
  B7P25_RS20880 (B7P25_20830) - 3871865..3872065 (+) 201 WP_000929284.1 hypothetical protein -
  B7P25_RS20885 (B7P25_20835) - 3872188..3873351 (+) 1164 WP_000434592.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=225555 B7P25_RS20860 WP_000811492.1 3868806..3869240(-) (nucA/comI) [Bacillus thuringiensis strain BM-BT15426]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=225555 B7P25_RS20860 WP_000811492.1 3868806..3869240(-) (nucA/comI) [Bacillus thuringiensis strain BM-BT15426]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment