Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   B7P25_RS08195 Genome accession   NZ_CP020723
Coordinates   1503666..1504268 (-) Length   200 a.a.
NCBI ID   WP_000155594.1    Uniprot ID   Q81SS2
Organism   Bacillus thuringiensis strain BM-BT15426     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1498666..1509268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7P25_RS08175 (B7P25_08155) dnaD 1498994..1499701 (+) 708 WP_000728549.1 DNA replication protein DnaD -
  B7P25_RS08180 (B7P25_08160) nth 1499717..1500364 (+) 648 WP_000933025.1 endonuclease III -
  B7P25_RS08185 (B7P25_08165) - 1500368..1500886 (+) 519 WP_087931515.1 YpoC family protein -
  B7P25_RS08190 (B7P25_08170) - 1500910..1503600 (-) 2691 WP_087931516.1 PBP1A family penicillin-binding protein -
  B7P25_RS08195 (B7P25_08175) recU 1503666..1504268 (-) 603 WP_000155594.1 Holliday junction resolvase RecU Machinery gene
  B7P25_RS08200 (B7P25_08180) - 1504372..1505397 (+) 1026 WP_087931517.1 DUF2515 domain-containing protein -
  B7P25_RS28900 (B7P25_08185) - 1505521..1505697 (-) 177 WP_001982922.1 hypothetical protein -
  B7P25_RS08210 (B7P25_08190) - 1505783..1506025 (+) 243 WP_001252043.1 hypothetical protein -
  B7P25_RS08215 (B7P25_08195) - 1506041..1506385 (+) 345 WP_087931518.1 YppE family protein -
  B7P25_RS08220 (B7P25_08200) - 1506411..1506602 (-) 192 WP_000242663.1 YppF family protein -
  B7P25_RS08225 (B7P25_08205) - 1506782..1507263 (+) 482 Protein_1468 YppG family protein -
  B7P25_RS08230 (B7P25_08210) - 1507419..1507844 (+) 426 WP_000546265.1 CotD family spore coat protein -
  B7P25_RS08235 (B7P25_08215) - 1507901..1508455 (+) 555 WP_000862920.1 DUF1273 domain-containing protein -
  B7P25_RS08240 (B7P25_08220) gpsB 1508553..1508891 (+) 339 WP_000622430.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23342.66 Da        Isoelectric Point: 9.5601

>NTDB_id=225547 B7P25_RS08195 WP_000155594.1 1503666..1504268(-) (recU) [Bacillus thuringiensis strain BM-BT15426]
MTIRYPNGKRYNQASQPHKTPIKKHTYSNRGMSLEEELNETNEYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVIAHNGIAFVIIKFTLFDELYLLDAKHIITFWNRQ
NTGGRKSITKEEIVEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=225547 B7P25_RS08195 WP_000155594.1 1503666..1504268(-) (recU) [Bacillus thuringiensis strain BM-BT15426]
ATGACCATTCGTTACCCAAATGGAAAACGGTACAATCAAGCTTCACAACCTCATAAAACACCAATTAAAAAACATACTTA
TAGTAACAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACAAATGAATATTACTTAACCCATAATATTGCATGTGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAGGCGTATTTT
AAACAACCTTCTACAACAGATTACAACGGTGTATATAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACAAAAAATAA
AACCAGCTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTAATTGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAACTTTATTTATTAGATGCAAAACATATTATTACATTTTGGAATCGTCAA
AATACTGGTGGACGAAAATCGATTACGAAAGAAGAAATAGTAGAGCATGGATCTTTATTATCATGCGGTTATCACCCTCG
GATTGATTATATCCGTGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81SS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

62.5

100

0.625

Multiple sequence alignment