Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   B7P25_RS06245 Genome accession   NZ_CP020723
Coordinates   1160120..1161946 (+) Length   608 a.a.
NCBI ID   WP_000003393.1    Uniprot ID   A0A0G8DIA9
Organism   Bacillus thuringiensis strain BM-BT15426     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1155120..1166946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7P25_RS06225 (B7P25_06210) - 1155443..1156117 (-) 675 WP_000362618.1 TerC family protein -
  B7P25_RS06230 (B7P25_06215) mecA 1156444..1157127 (+) 684 WP_000350710.1 adaptor protein MecA Regulator
  B7P25_RS06235 (B7P25_06220) - 1157200..1158744 (+) 1545 WP_000799201.1 cardiolipin synthase -
  B7P25_RS06240 (B7P25_06225) - 1158825..1160069 (+) 1245 WP_087931424.1 competence protein CoiA -
  B7P25_RS06245 (B7P25_06230) pepF 1160120..1161946 (+) 1827 WP_000003393.1 oligoendopeptidase F Regulator
  B7P25_RS28855 (B7P25_06235) - 1162066..1162239 (-) 174 WP_001984995.1 hypothetical protein -
  B7P25_RS06255 (B7P25_06240) - 1162469..1163362 (-) 894 WP_000360638.1 ClpXP adapter SpxH family protein -
  B7P25_RS06260 (B7P25_06245) - 1163362..1163760 (-) 399 WP_000043381.1 hypothetical protein -
  B7P25_RS06265 (B7P25_06250) - 1163941..1164519 (-) 579 WP_000191105.1 CYTH domain-containing protein -
  B7P25_RS06270 (B7P25_06255) - 1164666..1165037 (+) 372 WP_001180003.1 hypothetical protein -
  B7P25_RS06275 (B7P25_06260) - 1165068..1165706 (+) 639 WP_001081482.1 GTP pyrophosphokinase -
  B7P25_RS06280 (B7P25_06265) - 1165725..1166522 (+) 798 WP_000673194.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70334.62 Da        Isoelectric Point: 4.6458

>NTDB_id=225544 B7P25_RS06245 WP_000003393.1 1160120..1161946(+) (pepF) [Bacillus thuringiensis strain BM-BT15426]
MSEQNTAKTLPDRNEIEESSTWRLEDIFQTDTEWEKEFQAIKELLPKLTEFKGKLGDSANNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVYETYGKYKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLIESVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDMLLKSLHVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=225544 B7P25_RS06245 WP_000003393.1 1160120..1161946(+) (pepF) [Bacillus thuringiensis strain BM-BT15426]
ATGTCTGAACAAAATACAGCAAAAACATTACCAGATCGTAACGAGATTGAAGAATCAAGCACGTGGAGATTGGAAGATAT
TTTCCAAACGGATACAGAGTGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTACCGAAGTTAACTGAATTTAAAGGGA
AACTCGGTGACTCTGCGAACAATTTACTTGAAGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGTTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCGTTAAATGACCGCGCGACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCATATATTGTGCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTAA
AAGAAAATAGAGACTTAAGTGTATATGAACATGCATTAGAAGAAATTACGCGCCAACGTCCGCACGTATTATCTGAAGCG
GAAGAGGCTTTATTAGCAGAAGCATCTGAAGTAATGAGTGCGTCAAGTAATACATTTGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAAGTAGAGATTACACATGGTCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTGCGCGAAGATGCATTTAAAGCTGTATATGAAACGTATGGAAAATATAAGAATACATTTGCAAGTACG
TTAAGCGGAGCTGTGAAGCGTAATAATTTTAATGCGCGCGTTCGTAAATACGACTCTGCACGTCAAGCGGCATTAAGTAA
TAATAATATTCCTGAAGCAGTGTACGATCAACTTATTGAATCTGTAAATGATAATTTACACTTGTTGCATCGTTACATCG
ATATTCGTAAGCGTGCACTAGGGCTTGATGAGCTTCATATGTATGATTTATATACACCGCTTGTACCAGAAGTGAAAATG
AATGTGAAGTATGAAGAAGCGCAAGACATGTTATTAAAATCTTTACATGTACTTGGTGATGAGTATGTTGATATTTTAAA
AGAAGCATATGAAAATCGTTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGAGCATATTCATCTGGTGCATATG
GAACAAATCCTTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAATTTGGTCATTCT
GTGCATAGTTACTATACAAGAAAAACGCAGCCGCACGTATACGGTGACTATTCAATCTTTGTTGCGGAAGTAGCATCAAC
CTGTAATGAGGCGTTGCTAAACGATTACTTATTAAAAACGACAGAAGATAAGAAAGAACGACTATACTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACGATGTTCGCAGAGTTTGAGCATATTATTCATAAGAAAGTACAA
GAGGGACATGCGGTTACGCCAGACATGTTAACGGAGATCTACTATGATTTAAATAAGAAATATTTCGGTGATGCTTTAGT
AATTGATGAAGAAATTGGTTTAGAGTGGTCACGTATTCCGCACTTCTACTACAACTATTACGTATACCAATACGCAACAG
GATTTAGTGCAGCAACTGCTCTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGCTATATTAATGAGTTC
TTAAAGGCAGGAAGTTCGGATTATCCAATTGAAGTGCTGAAAAAAGCTGGAGTAGATATGGCATCTCCAGAACCGGTAAA
AGAAGCATTGCAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAGAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8DIA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.084

97.533

0.488

Multiple sequence alignment