Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B7P25_RS00550 Genome accession   NZ_CP020723
Coordinates   98618..99994 (+) Length   458 a.a.
NCBI ID   WP_087931155.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain BM-BT15426     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 93618..104994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7P25_RS00530 (B7P25_00530) ctsR 93816..94277 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  B7P25_RS00535 (B7P25_00535) - 94449..94994 (+) 546 Protein_75 UvrB/UvrC motif-containing protein -
  B7P25_RS00540 (B7P25_00540) - 94999..96063 (+) 1065 WP_000050832.1 protein arginine kinase -
  B7P25_RS00545 (B7P25_00545) clpC 96086..98521 (+) 2436 WP_087931154.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  B7P25_RS00550 (B7P25_00550) radA 98618..99994 (+) 1377 WP_087931155.1 DNA repair protein RadA Machinery gene
  B7P25_RS00555 (B7P25_00555) disA 99998..101071 (+) 1074 WP_087931156.1 DNA integrity scanning diadenylate cyclase DisA -
  B7P25_RS00560 (B7P25_00560) - 101232..102342 (+) 1111 Protein_80 PIN/TRAM domain-containing protein -
  B7P25_RS00565 (B7P25_00565) ispD 102359..103039 (+) 681 WP_087931157.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  B7P25_RS00570 (B7P25_00570) ispF 103156..103632 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49888.53 Da        Isoelectric Point: 7.1422

>NTDB_id=225532 B7P25_RS00550 WP_087931155.1 98618..99994(+) (radA) [Bacillus thuringiensis strain BM-BT15426]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=225532 B7P25_RS00550 WP_087931155.1 98618..99994(+) (radA) [Bacillus thuringiensis strain BM-BT15426]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAATCACCAAAATATATGGGGAAATGTCCAGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCCGTTGTATCATCAAGACGCCTAAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACAAAATTCCAAGAATTT
AACCGTGTACTAGGTGGCGGAATTGTAGATGGATCCTTAGTACTTATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCAGATGCTTCATATGATGTACTATATATATCAGGTGAGGAATCAGCAAAACAGA
TTAAACTGCGTGCAGATCGTCTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACAGATTTACAGCGAATTGCA
ACACACATTGAAGAGATGAACCCAGCCTTTGTTGTTATTGACTCTATTCAAACAATACATTTACCTGAAGTAACATCAGC
GCCGGGAAGTGTGGCACAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACAAAAGGAATCCCAATTTTCA
TCGTAGGACATGTGACAAAAGAAGGAGCAATTGCAGGACCGCGTATGCTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGACACCATACATACCGTATTTTACGAGCAGTAAAGAATCGCTTTGGTTCTACGAATGAAATGGGTATCTT
TGAAATGAAAGAACTTGGCCTTGCAGAAGTATTAAACCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTGGGAGTTGCAG
GATCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACAGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTACTAGAAAAAAGAACAGG
CTTACTATTACAAAATCAAGACGCATATTTAAAAGTAGCAGGTGGTTTGAAATTAGATGAACCAGCAATTGATTTAGCTG
TCGCCTTAAGTATAGCTTCAAGTTTCAGAGATAAATCTACAGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATCATTCC
TAGAAAAAATTTAGGAGGATGGACAATTCCAGAGGGGATTGAGGTAGTAGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629

Multiple sequence alignment