Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   B7474_RS02230 Genome accession   NZ_CP020714
Coordinates   461477..462451 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain K18     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 456477..467451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7474_RS02190 (B7474_02180) gcvT 456978..458069 (-) 1092 WP_000093348.1 glycine cleavage system aminomethyltransferase GcvT -
  B7474_RS02195 (B7474_02185) - 458228..458752 (-) 525 WP_001015120.1 shikimate kinase -
  B7474_RS02200 (B7474_02190) - 458742..458888 (-) 147 WP_001792109.1 hypothetical protein -
  B7474_RS02205 (B7474_02195) comGF 458985..459482 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  B7474_RS02210 (B7474_02200) comGE 459400..459699 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  B7474_RS02215 (B7474_02205) comGD 459686..460132 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  B7474_RS02220 (B7474_02210) comGC 460110..460421 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  B7474_RS02225 (B7474_02215) comGB 460435..461505 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  B7474_RS02230 (B7474_02220) comGA 461477..462451 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  B7474_RS02235 (B7474_02225) - 462503..463126 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  B7474_RS02240 (B7474_02230) - 463123..463452 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  B7474_RS02245 (B7474_02235) - 463452..464438 (-) 987 WP_000161314.1 ROK family glucokinase -
  B7474_RS02250 (B7474_02240) - 464435..464638 (-) 204 WP_000087561.1 YqgQ family protein -
  B7474_RS02255 (B7474_02245) - 464619..466082 (-) 1464 WP_001017858.1 rhomboid family protein -
  B7474_RS02260 (B7474_02250) - 466094..466633 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  B7474_RS02265 (B7474_02255) rpmG 466842..466991 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=225489 B7474_RS02230 WP_000697220.1 461477..462451(-) (comGA) [Staphylococcus aureus strain K18]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=225489 B7474_RS02230 WP_000697220.1 461477..462451(-) (comGA) [Staphylococcus aureus strain K18]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment