Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C2R21_RS00540 Genome accession   NZ_CP026342
Coordinates   117109..117549 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 9FA     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112109..122549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2R21_RS00520 coaE 112599..113219 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  C2R21_RS24305 - 113244..113288 (+) 45 WP_120795372.1 protein YacM -
  C2R21_RS00525 guaC 113444..114487 (+) 1044 WP_001217338.1 GMP reductase -
  C2R21_RS00530 hofC 114522..115724 (-) 1203 WP_000157266.1 protein transport protein HofC -
  C2R21_RS00535 pilB 115714..117099 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  C2R21_RS00540 pilA 117109..117549 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  C2R21_RS00550 nadC 117752..118645 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C2R21_RS00555 ampD 118733..119284 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C2R21_RS00560 ampE 119281..120135 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  C2R21_RS00565 aroP 120178..121551 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=225429 C2R21_RS00540 WP_000360904.1 117109..117549(-) (pilA) [Escherichia coli strain 9FA]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=225429 C2R21_RS00540 WP_000360904.1 117109..117549(-) (pilA) [Escherichia coli strain 9FA]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment