Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B5D77_RS16110 Genome accession   NZ_CP020664
Coordinates   3157467..3158993 (-) Length   508 a.a.
NCBI ID   WP_008206299.1    Uniprot ID   A0A2P1UI87
Organism   Microcystis sp. MC19     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3152467..3163993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D77_RS16075 (B5D77_15875) - 3153448..3154278 (-) 831 WP_106909192.1 Uma2 family endonuclease -
  B5D77_RS16080 (B5D77_15880) - 3154464..3155393 (-) 930 WP_008206313.1 YbhN family protein -
  B5D77_RS16085 (B5D77_15885) - 3155386..3155844 (-) 459 WP_106909193.1 DUF29 domain-containing protein -
  B5D77_RS16090 (B5D77_15890) - 3155856..3156311 (-) 456 WP_008206307.1 DUF29 domain-containing protein -
  B5D77_RS16095 (B5D77_15895) - 3156324..3156779 (-) 456 WP_106909194.1 DUF29 domain-containing protein -
  B5D77_RS16100 (B5D77_15900) - 3156792..3157247 (-) 456 WP_106909195.1 DUF29 domain-containing protein -
  B5D77_RS16105 (B5D77_15905) petL 3157320..3157421 (-) 102 WP_002791324.1 cytochrome b6-f complex subunit PetL -
  B5D77_RS16110 (B5D77_15910) radA 3157467..3158993 (-) 1527 WP_008206299.1 DNA repair protein RadA Machinery gene
  B5D77_RS16115 (B5D77_15915) rpaB 3159227..3159955 (+) 729 WP_002734727.1 response regulator transcription factor RpaB -
  B5D77_RS16120 (B5D77_15920) - 3159965..3160606 (+) 642 WP_008206296.1 cofactor assembly of complex C subunit B -
  B5D77_RS16125 (B5D77_15925) - 3161272..3161490 (+) 219 WP_002746174.1 DUF433 domain-containing protein -
  B5D77_RS16130 (B5D77_15930) - 3161492..3161794 (+) 303 WP_106909196.1 DUF5615 family PIN-like protein -
  B5D77_RS16135 (B5D77_15935) - 3161927..3162307 (-) 381 WP_106909197.1 hypothetical protein -
  B5D77_RS16140 (B5D77_15940) - 3162483..3162983 (+) 501 WP_106910014.1 hypothetical protein -
  B5D77_RS27325 - 3162998..3163180 (-) 183 WP_238560447.1 hypothetical protein -

Sequence

Protein


Download         Length: 508 a.a.        Molecular weight: 54893.35 Da        Isoelectric Point: 4.9634

>NTDB_id=225344 B5D77_RS16110 WP_008206299.1 3157467..3158993(-) (radA) [Microcystis sp. MC19]
MAKSRTIYICSACGAESPQWLGKCPSCDTYGTLEEQVVAGGNNQAVNRPGWQNSRPNNGKGQRNPQPRISVRFSEITQYE
QERFPSGYGEFDRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVTNQLAQRLPRILYVSAEESGQQIKLRAARLGVGVVAVE
SENNGNGKEKKASESTAELDNHLYVLPETDLEEILRELESLRPQVAVIDSIQTLYFGALTSAPGSVAQVRECTSALMQVA
KRENITLLIVGHVTKEGAIAGPRVLEHLVDTVLYFEGDRYASHRLLRSVKNRFGATHEIGIFEMVDHGLVEVENPSELFL
GNRDEFSPGTATVVACEGTRPIVVELQALVSPTSYASPRRSTTGIEYNRLQQILAVLEKRVGIPLSKLDAYVASAGGLGV
EEPAADLGVAIAVVASFRDRVVDPRTVLIGEVGLGGQVRLVSQMELRLKEAAKLGFKRAIVPRGQVYPEDVGLEIVPVGK
VIEAIVAAIPPQQRFGEDPEDEEGEGQE

Nucleotide


Download         Length: 1527 bp        

>NTDB_id=225344 B5D77_RS16110 WP_008206299.1 3157467..3158993(-) (radA) [Microcystis sp. MC19]
ATGGCGAAATCGCGAACAATATATATCTGTAGTGCTTGTGGGGCGGAATCTCCCCAATGGTTGGGAAAATGCCCCAGTTG
TGACACCTACGGGACTTTAGAAGAACAAGTGGTAGCTGGTGGCAATAATCAGGCGGTAAATCGCCCAGGATGGCAAAATA
GTCGCCCAAATAACGGCAAAGGACAACGCAACCCGCAGCCGCGCATCTCGGTGCGCTTCTCGGAAATTACCCAGTATGAA
CAGGAACGTTTTCCCTCTGGGTACGGGGAATTTGATCGCGTTCTCGGTGGTGGCATTGTCCCCGGTTCTCTTGTACTAAT
TGGCGGCGATCCGGGTATCGGCAAATCGACGCTTTTACTGCAAGTTACCAACCAACTCGCCCAAAGATTACCGCGCATCC
TCTACGTTTCCGCCGAGGAATCGGGACAACAGATTAAACTGCGGGCTGCCCGTTTGGGGGTGGGAGTGGTTGCCGTGGAG
TCGGAAAATAACGGCAATGGCAAGGAGAAAAAAGCCTCCGAATCAACCGCAGAACTCGATAATCATCTCTACGTTTTGCC
AGAAACCGATTTAGAGGAAATTTTGCGAGAATTAGAATCTCTACGCCCACAAGTGGCGGTAATTGACAGTATTCAAACTC
TTTATTTTGGGGCCTTAACTTCCGCGCCGGGATCCGTCGCCCAAGTTCGCGAATGTACCTCCGCCTTGATGCAGGTGGCA
AAACGGGAGAATATTACTTTATTAATTGTCGGTCATGTTACCAAAGAAGGGGCGATCGCTGGTCCGCGAGTTTTGGAACA
TTTGGTCGATACAGTGTTATATTTTGAAGGCGATCGCTATGCCTCCCATCGTCTCTTAAGATCCGTGAAAAACCGTTTTG
GGGCAACCCATGAGATCGGTATTTTCGAGATGGTGGATCACGGTTTGGTGGAAGTGGAAAACCCCTCAGAATTGTTTTTA
GGCAATCGCGACGAATTTTCCCCGGGTACGGCGACGGTGGTAGCCTGTGAAGGTACACGCCCGATTGTGGTGGAGTTACA
GGCATTGGTTAGCCCTACCAGCTATGCTTCCCCGCGAAGATCGACTACTGGCATAGAATACAACCGATTACAGCAAATTC
TCGCCGTTTTAGAAAAGCGCGTCGGCATTCCTTTATCGAAATTAGATGCCTATGTTGCCTCCGCAGGAGGGTTGGGAGTG
GAAGAACCGGCGGCGGATCTGGGAGTTGCGATCGCTGTTGTCGCCAGTTTTCGCGATCGAGTCGTGGATCCGCGCACAGT
TTTAATCGGGGAAGTGGGATTAGGCGGACAAGTCCGCTTAGTGTCGCAAATGGAACTAAGATTAAAAGAAGCGGCGAAAT
TGGGCTTTAAACGGGCGATTGTCCCCAGAGGTCAAGTTTATCCCGAAGATGTGGGCTTAGAAATTGTTCCAGTCGGCAAG
GTAATCGAGGCAATTGTCGCCGCAATTCCCCCCCAGCAGCGCTTTGGGGAAGATCCGGAAGACGAGGAAGGGGAAGGGCA
AGAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(401-463)

KaiC (ATPase)

(85-296)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P1UI87

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.511

96.26

0.486
  radA Streptococcus mitis SK321

46.555

94.291

0.439
  radA Streptococcus pneumoniae D39

47.21

91.732

0.433
  radA Streptococcus pneumoniae TIGR4

47.21

91.732

0.433
  radA Streptococcus mitis NCTC 12261

47.21

91.732

0.433
  radA Streptococcus pneumoniae R6

47.21

91.732

0.433
  radA Streptococcus pneumoniae Rx1

47.21

91.732

0.433

Multiple sequence alignment