Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VV1_RS06560 Genome accession   NC_004459
Coordinates   1365125..1366909 (+) Length   594 a.a.
NCBI ID   WP_011079356.1    Uniprot ID   A0A3Q0L3M5
Organism   Vibrio vulnificus CMCP6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1360125..1371909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VV1_RS06540 (VV1_1377) pilM 1362439..1363446 (+) 1008 WP_011079352.1 type IV pilus assembly protein PilM Machinery gene
  VV1_RS06545 (VV1_1378) pilN 1363436..1364026 (+) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  VV1_RS06550 (VV1_1379) pilO 1364019..1364609 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  VV1_RS06555 (VV1_1380) pilP 1364599..1365111 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  VV1_RS06560 (VV1_1381) pilQ 1365125..1366909 (+) 1785 WP_011079356.1 type IV pilus secretin PilQ family protein Machinery gene
  VV1_RS06565 (VV1_1382) aroK 1367111..1367629 (+) 519 WP_011079357.1 shikimate kinase AroK -
  VV1_RS06570 (VV1_1383) aroB 1367662..1368753 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -
  VV1_RS06575 (VV1_1384) - 1368804..1370300 (+) 1497 WP_011079359.1 AAA family ATPase -
  VV1_RS06580 (VV1_1385) - 1370380..1371207 (+) 828 WP_011079360.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64520.62 Da        Isoelectric Point: 4.5316

>NTDB_id=22531 VV1_RS06560 WP_011079356.1 1365125..1366909(+) (pilQ) [Vibrio vulnificus CMCP6]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGSEGGSASIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=22531 VV1_RS06560 WP_011079356.1 1365125..1366909(+) (pilQ) [Vibrio vulnificus CMCP6]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACCTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAACGCTG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACGGTGGTGGATTTGAAACCGGTAGCGGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTCAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTCTCGACCGTGGTTGAAAG
TATCGAAGTGTTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTTAGTGGTGAATTTCGTCACGACTATCGTCTGT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAACAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGTTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAATGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCCGTGACTGGTAATGTGACGTTGAGATTGGATGGCGTACCTTGGCAGCAAGTGCTCGATATCA
TTCTTCAAGTGAAAGGTTTAGATAAACGTGTTGAAGGCAATGTTGTGCTCATCGCTCCTCAAGATGAATTGGATTTGAGA
GAGAAGCAGCAGCTTGAGAAAGAGCGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATCAAAGTCAACTTTGC
CAAAGCGTCAGACATCGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCGCGAATTGTTACGGTGAACGAAGGCAATCTTGATGAGCTTGGTGTACGTTG
GGGTTTCACCTCCATCAACGGCAGCACAACTGTCGGTGGTTCAATCGAAAACAACCTTGCAACCATTGGTCTCTACAATG
CAGGTAGTGGTAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGCAGTGAAGGCGGTTCGGCCTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACCTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAACTCTCTGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACGGAGATCCCTTATCTAGAGTCTTCCTCCAGTGGAGCTACCACGGTTTCATTT
AAGAAAGCCGTGCTTAGCCTGAAAGTGACCCCACAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCCGGCGAAATTGTCAAAACAGGCACTGGAGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGTATTACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCTTTATTGGGGGCGCTTTTCCGCCGTAGCTATGAAAACGTGGGTAAACGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.729

99.327

0.732
  pilQ Vibrio cholerae strain A1552

73.729

99.327

0.732
  pilQ Legionella pneumophila strain ERS1305867

41.156

96.128

0.396
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

39.539

94.949

0.375
  comQ Acinetobacter baylyi ADP1

37.162

99.663

0.37

Multiple sequence alignment