Detailed information NTG0224809

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B7L46_RS13435
• Genome accession: NZ_CP020597
• Coordinates: 2594381..2595499 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain HWBA8
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2589381..2600499

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L46_RS13400 (B7L46_13400)	-	2590211..2591059 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
B7L46_RS13405 (B7L46_13405)	-	2591311..2591766 (+)	456	WP_000782976.1	bacteriohemerythrin	-
B7L46_RS13415 (B7L46_13415)	-	2591919..2592986 (+)	1068	WP_004736014.1	hypothetical protein	-
B7L46_RS13420 (B7L46_13420)	-	2592986..2593309 (+)	324	WP_000442582.1	RnfH family protein	-
B7L46_RS13425 (B7L46_13425)	-	2593364..2593762 (-)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
B7L46_RS13430 (B7L46_13430)	fur	2593874..2594311 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
B7L46_RS13435 (B7L46_13435)	pilU	2594381..2595499 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B7L46_RS13440 (B7L46_13440)	pilT	2595527..2596564 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
B7L46_RS13445 (B7L46_13445)	-	2596691..2597383 (+)	693	WP_001108513.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=224809 B7L46_RS13435 WP_000347039.1 2594381..2595499(-) (pilU) [Acinetobacter baumannii strain HWBA8]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=224809 B7L46_RS13435 WP_000347039.1 2594381..2595499(-) (pilU) [Acinetobacter baumannii strain HWBA8]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395