Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   B7L44_RS18110 Genome accession   NZ_CP020588
Coordinates   3704714..3705481 (-) Length   255 a.a.
NCBI ID   WP_002053237.1    Uniprot ID   A0A2L1VK05
Organism   Acinetobacter nosocomialis strain SSA3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3699714..3710481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7L44_RS18090 (B7L44_18090) - 3700779..3701477 (-) 699 WP_002053293.1 YfgM family protein -
  B7L44_RS18095 (B7L44_18095) hisS 3701506..3702798 (-) 1293 WP_083009233.1 histidine--tRNA ligase -
  B7L44_RS18100 (B7L44_18100) ispG 3702795..3703910 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  B7L44_RS18105 (B7L44_18105) - 3703932..3704723 (-) 792 WP_002053491.1 helix-turn-helix domain-containing protein -
  B7L44_RS18110 (B7L44_18110) pilF 3704714..3705481 (-) 768 WP_002053237.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  B7L44_RS18115 (B7L44_18115) rlmN 3705530..3706762 (-) 1233 WP_083009234.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  B7L44_RS18120 (B7L44_18120) ndk 3706885..3707316 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  B7L44_RS18125 (B7L44_18125) iscX 3707430..3707630 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  B7L44_RS18130 (B7L44_18130) - 3707730..3708281 (-) 552 WP_004710660.1 phosphatase PAP2 family protein -
  B7L44_RS18135 (B7L44_18135) - 3708306..3709604 (-) 1299 WP_083009236.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 28806.75 Da        Isoelectric Point: 8.7393

>NTDB_id=224566 B7L44_RS18110 WP_002053237.1 3704714..3705481(-) (pilF) [Acinetobacter nosocomialis strain SSA3]
MGVAVACLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGILLQQEGSKPNLE
KAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDIASAEKTFKQAL
LANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQLRALFPESPEY
QRYLQLQYSTEAVWK

Nucleotide


Download         Length: 768 bp        

>NTDB_id=224566 B7L44_RS18110 WP_002053237.1 3704714..3705481(-) (pilF) [Acinetobacter nosocomialis strain SSA3]
ATGGGGGTTGCTGTTGCATGTCTGGCCAGTGGTTGTCAGTCTTCGCCAACGCTTAAAAAGGACCCGGAAAAAGCAGTTAA
GGTGCGTACGCAGCTTGCCGCAGAATACATTCGTTCTGGTGACTTGGACTCTGCAAAGCGTTCCCTTGACCAAGCTTTAA
GTGTCGATAGCCGTGATGCAACGGCAAATATGATGATGGGCATTTTATTGCAACAAGAAGGCAGTAAACCCAATTTAGAA
AAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAATGCTCAGGCGCGTAATAATTATGGTACGTATTT
GTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTGCTGGTGCAACATTAGGTTATGATCAACGCTATC
AAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATTGCGAGTGCTGAAAAAACATTCAAACAAGCACTC
CTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAATTTTTTATTTGCAGCAGCAGATTCCGGCTGCTAC
ACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGGGTGCTCGCGCACTTTGGATTGGTTTAAGAGTGG
CTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAGTTACGTGCTTTATTCCCGGAAAGTCCAGAATAT
CAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(28-57)

Tetratricopeptide repeat (TPR_16)

(78-123)

Tetratricopeptide repeat (TPR_16)

(138-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1VK05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.431

100

0.984
  pilF Acinetobacter baylyi ADP1

75.494

99.216

0.749

Multiple sequence alignment