Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   B7D75_RS22955 Genome accession   NZ_CP020560
Coordinates   4840018..4841511 (+) Length   497 a.a.
NCBI ID   WP_034080943.1    Uniprot ID   A0A2R3IXQ9
Organism   Pseudomonas paraeruginosa strain Cr1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4835018..4846511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7D75_RS22925 (B7D75_22940) - 4835233..4836042 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  B7D75_RS22930 (B7D75_22945) kdsD 4836452..4837432 (+) 981 WP_170870649.1 arabinose-5-phosphate isomerase KdsD -
  B7D75_RS22935 (B7D75_22950) - 4837432..4837971 (+) 540 WP_107114451.1 HAD family hydrolase -
  B7D75_RS22940 (B7D75_22955) lptC 4837980..4838552 (+) 573 WP_003155089.1 LPS export ABC transporter periplasmic protein LptC -
  B7D75_RS22945 (B7D75_22960) lptA 4838539..4839066 (+) 528 WP_012077214.1 lipopolysaccharide transport periplasmic protein LptA -
  B7D75_RS22950 (B7D75_22965) lptB 4839066..4839791 (+) 726 WP_003155093.1 LPS export ABC transporter ATP-binding protein -
  B7D75_RS22955 (B7D75_22970) rpoN/rpoN1 4840018..4841511 (+) 1494 WP_034080943.1 RNA polymerase factor sigma-54 Machinery gene
  B7D75_RS22960 (B7D75_22975) hpf 4841589..4841897 (+) 309 WP_003155096.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  B7D75_RS22965 (B7D75_22980) ptsN 4841911..4842375 (+) 465 WP_003155098.1 PTS IIA-like nitrogen regulatory protein PtsN -
  B7D75_RS22970 (B7D75_22985) rapZ 4842377..4843237 (+) 861 WP_003155100.1 RNase adapter RapZ -
  B7D75_RS22975 (B7D75_22990) - 4843267..4843539 (+) 273 WP_003155102.1 HPr family phosphocarrier protein -
  B7D75_RS22980 (B7D75_22995) - 4843611..4844513 (-) 903 WP_034080945.1 ZIP family metal transporter -
  B7D75_RS22985 (B7D75_23000) - 4844580..4845194 (-) 615 WP_034080946.1 superoxide dismutase -
  B7D75_RS22990 (B7D75_23005) - 4845207..4845656 (-) 450 WP_012077234.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55940.15 Da        Isoelectric Point: 4.4128

>NTDB_id=224128 B7D75_RS22955 WP_034080943.1 4840018..4841511(+) (rpoN/rpoN1) [Pseudomonas paraeruginosa strain Cr1]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDGSDPLADGAEQATSAPQESALQE
STTPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSSDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAALDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPPATPWLDEALRLV
SDYLDLLGGRDYGQLMRRMKLKEDELRLVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=224128 B7D75_RS22955 WP_034080943.1 4840018..4841511(+) (rpoN/rpoN1) [Pseudomonas paraeruginosa strain Cr1]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCTCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTCTCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACGGCAGCGACCCGCTCGCCGATGGCGCCGAACAGGCAACCAGCGCGCCGCAGGAGAGCGCCCTCCAGGAA
AGCACCACGCCCAGCGTAGAAAGCCTCGACGACGACCAGTGGAGCGAGCGCATCCCCAGCGAACTGCCGGTGGATACCGC
CTGGGAGGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAGCGACGACGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTGAACCTGGCGCCGATGTCGGACACCGACCGCATGATCGCG
GTGACCATCATCGACAGCATCAACAATGACGGCTATCTGGAGGAGTCCCTCGAGGAGATCCTCGCCGCCCTCGATCCGGA
GCTGGACGTCGAGCTCGACGAAGTCGAAGTCGTGCTGCGCCGCATCCAGCAGTTGGAGCCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGTTGCTGCAACTGCGCCAGCTGCCACCCGCCACGCCCTGGCTGGACGAAGCCCTGCGCCTGGTT
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACGGCCAGTTGATGCGGCGCATGAAGCTCAAGGAAGACGAGTTGCG
CCTGGTCATCGAGTTGATCCAGTGCCTGCATCCACGGCCCGGCTCGCAGATCGAGTCGAGCGAGGCCGAATACATCGTCC
CGGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTGGAACTGAACCAGGAGGCGATGCCACGCCTGCGTGTCAAC
GCCACCTATGCCGGCATGGTCCGCCGTGCCGACAGCAGTGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCGCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAGACCCTGATGAAGGTGGCGACGCAGATCGTCGAACACCAGCGTGGCT
TTCTCGACTACGGCGAGGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAGTCGACC
ATTTCGCGGGTCACCACGCAGAAGTACATGCATACGCCGCGCGGTATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGCACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAG
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.785

100

0.481

Multiple sequence alignment