Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CHELV3228_RS02565 Genome accession   NZ_CP020478
Coordinates   485499..486443 (-) Length   314 a.a.
NCBI ID   WP_082199400.1    Uniprot ID   A0AAX2UK20
Organism   Campylobacter helveticus strain ATCC 51209     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 486493..508561 485499..486443 flank 50


Gene organization within MGE regions


Location: 485499..508561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHELV3228_RS02565 (CHELV3228_0509) waaF 485499..486443 (-) 945 WP_082199400.1 lipopolysaccharide heptosyltransferase II Regulator
  CHELV3228_RS02570 (CHELV3228_0510) - 486493..487353 (+) 861 WP_082199401.1 glycosyltransferase family 2 protein -
  CHELV3228_RS02575 (CHELV3228_0511) rfbA 487350..488237 (+) 888 WP_082199402.1 glucose-1-phosphate thymidylyltransferase RfbA -
  CHELV3228_RS02580 (CHELV3228_0512) rfbB 488234..489256 (+) 1023 WP_082199403.1 dTDP-glucose 4,6-dehydratase -
  CHELV3228_RS10195 - 489253..489483 (+) 231 WP_167562782.1 hypothetical protein -
  CHELV3228_RS10200 - 489480..489836 (+) 357 WP_167562783.1 acyltransferase -
  CHELV3228_RS02590 (CHELV3228_0514) - 489839..490273 (+) 435 WP_082199404.1 FdtA/QdtA family cupin domain-containing protein -
  CHELV3228_RS02595 (CHELV3228_0515) - 490276..490668 (+) 393 WP_082199405.1 FdtA/QdtA family cupin domain-containing protein -
  CHELV3228_RS02600 (CHELV3228_0516) - 490668..491204 (+) 537 WP_082199406.1 acyltransferase -
  CHELV3228_RS02605 (CHELV3228_0517) - 491201..492214 (+) 1014 WP_082199407.1 GNAT family N-acetyltransferase -
  CHELV3228_RS02610 (CHELV3228_0518) - 492215..493015 (+) 801 WP_082199408.1 formyltransferase family protein -
  CHELV3228_RS02615 - 493012..493647 (+) 636 WP_082199409.1 glycosyltransferase family 2 protein -
  CHELV3228_RS02620 - 493640..494032 (+) 393 WP_141081303.1 hypothetical protein -
  CHELV3228_RS02625 (CHELV3228_0520) - 493989..495062 (+) 1074 WP_082199411.1 glycosyltransferase -
  CHELV3228_RS02630 (CHELV3228_0521) - 495072..496166 (+) 1095 WP_082199412.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  CHELV3228_RS02635 (CHELV3228_0522) - 496166..497350 (+) 1185 WP_082199413.1 glycosyltransferase family 8 protein -
  CHELV3228_RS02640 (CHELV3228_0523) murJ 497408..498862 (+) 1455 WP_082199414.1 murein biosynthesis integral membrane protein MurJ -
  CHELV3228_RS02645 (CHELV3228_0524) cysS 498849..500219 (+) 1371 WP_082199415.1 cysteine--tRNA ligase -
  CHELV3228_RS02650 (CHELV3228_0525) - 500219..501937 (+) 1719 WP_082200732.1 ABC transporter ATP-binding protein -
  CHELV3228_RS02655 (CHELV3228_0526) - 501976..503031 (+) 1056 WP_082199416.1 quinone-dependent dihydroorotate dehydrogenase -
  CHELV3228_RS02660 (CHELV3228_0527) - 503028..504278 (+) 1251 WP_082199417.1 pitrilysin family protein -
  CHELV3228_RS02665 (CHELV3228_0528) dapA 504271..505167 (+) 897 WP_082199418.1 4-hydroxy-tetrahydrodipicolinate synthase -
  CHELV3228_RS02670 (CHELV3228_0529) - 505167..505946 (+) 780 WP_082199419.1 enoyl-ACP reductase -
  CHELV3228_RS02675 (CHELV3228_0530) - 505962..507230 (-) 1269 WP_082199420.1 M20/M25/M40 family metallo-hydrolase -
  CHELV3228_RS02680 (CHELV3228_0531) - 507288..507806 (+) 519 WP_082199421.1 thermonuclease family protein -
  CHELV3228_RS10205 (CHELV3228_0532) - 507814..507987 (+) 174 WP_167562784.1 hypothetical protein -
  CHELV3228_RS02685 (CHELV3228_0533) - 507989..508561 (-) 573 WP_082199422.1 hypothetical protein -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35655.47 Da        Isoelectric Point: 10.5536

>NTDB_id=223614 CHELV3228_RS02565 WP_082199400.1 485499..486443(-) (waaF) [Campylobacter helveticus strain ATCC 51209]
MKIFINLPTWLGDCVMASAAIYGIKERFKEAEFVFFGSFVATGLFKEFPNSSIILEDKKKRYKQAFHLRQNHQFDLAFSF
RSALSAKLILKIIKSKKKFCFNKKILKDSHQVLKYLNFIEHTLNFQISSKDLKLPIKTKFKAPLQLKSGKKILALNPGAS
YGSAKRWDEEYFAKVGFEFAKSHEIIILGAGKEEAIICARIANILAQKGVRAKNLCNKTSIQTLAQNIALSDIFLTNDSG
AMHIGAALKVKMLVIFGPTKFTQTSPWQSENAKIIHLNLACMPCMKRTCILKHHKCMKDLSPKMVIEKLKEFKI

Nucleotide


Download         Length: 945 bp        

>NTDB_id=223614 CHELV3228_RS02565 WP_082199400.1 485499..486443(-) (waaF) [Campylobacter helveticus strain ATCC 51209]
ATGAAAATTTTTATCAATCTTCCCACTTGGCTAGGCGATTGCGTTATGGCTAGTGCGGCGATTTATGGCATTAAAGAGCG
GTTTAAAGAAGCGGAATTTGTCTTTTTTGGCTCTTTTGTAGCGACAGGGCTTTTTAAAGAATTTCCCAACTCAAGCATTA
TCTTAGAAGATAAAAAAAAGCGCTACAAACAAGCCTTTCATTTGCGTCAAAATCATCAATTTGACCTTGCATTTTCTTTT
CGCTCGGCACTTTCGGCAAAGCTTATTTTAAAAATCATAAAATCCAAAAAAAAATTTTGTTTCAACAAAAAAATCTTAAA
AGACTCACATCAAGTTTTAAAATACTTAAATTTCATTGAGCATACTTTAAATTTTCAAATCTCATCAAAAGATTTAAAAC
TACCCATCAAAACGAAATTTAAAGCACCCCTACAACTTAAAAGTGGCAAAAAAATTCTCGCTTTAAACCCTGGTGCAAGC
TATGGGAGTGCTAAACGCTGGGATGAAGAGTATTTTGCAAAAGTGGGGTTTGAATTTGCCAAAAGCCACGAAATTATTAT
TCTTGGGGCGGGTAAAGAAGAGGCTATCATTTGTGCTAGAATTGCCAACATACTCGCACAAAAAGGCGTTAGGGCGAAAA
ATTTATGCAACAAAACAAGCATTCAAACCCTCGCACAAAATATCGCCTTAAGTGATATTTTCCTCACAAATGACAGCGGA
GCCATGCACATAGGTGCGGCTTTAAAGGTGAAAATGCTAGTGATTTTTGGACCGACTAAATTCACTCAAACCTCACCTTG
GCAGAGCGAAAATGCTAAAATTATCCATCTTAATTTAGCTTGTATGCCCTGTATGAAACGCACCTGCATACTAAAACACC
ATAAATGTATGAAAGATTTAAGTCCTAAAATGGTGATAGAAAAATTGAAAGAATTTAAGATTTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(103-293)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.344

99.045

0.627

Multiple sequence alignment