Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B5S50_RS10070 Genome accession   NZ_CP020473
Coordinates   2177139..2178494 (-) Length   451 a.a.
NCBI ID   WP_217303703.1    Uniprot ID   -
Organism   Clostridium sp. 001     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2172139..2183494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5S50_RS10050 (B5S50_09970) ispD 2173490..2174182 (-) 693 WP_065078560.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  B5S50_RS10055 (B5S50_09975) - 2174186..2175313 (-) 1128 WP_065078561.1 PIN/TRAM domain-containing protein -
  B5S50_RS10060 (B5S50_09980) - 2175616..2176020 (+) 405 WP_013240714.1 hypothetical protein -
  B5S50_RS10065 (B5S50_09985) disA 2176060..2177124 (-) 1065 WP_065078582.1 DNA integrity scanning diadenylate cyclase DisA -
  B5S50_RS10070 (B5S50_09990) radA 2177139..2178494 (-) 1356 WP_217303703.1 DNA repair protein RadA Machinery gene
  B5S50_RS10075 (B5S50_09995) - 2178780..2181221 (-) 2442 WP_065078563.1 ATP-dependent Clp protease ATP-binding subunit -
  B5S50_RS10080 (B5S50_10000) - 2181242..2182282 (-) 1041 WP_065078564.1 protein arginine kinase -
  B5S50_RS10085 (B5S50_10005) - 2182285..2182833 (-) 549 WP_065078565.1 UvrB/UvrC motif-containing protein -
  B5S50_RS10090 (B5S50_10010) - 2182907..2183389 (-) 483 WP_065078566.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 49505.35 Da        Isoelectric Point: 8.0691

>NTDB_id=223581 B5S50_RS10070 WP_217303703.1 2177139..2178494(-) (radA) [Clostridium sp. 001]
MVKKKTVFVCQECGYESPKWLGKCPNCNKWNCMVEEIKSPQEVTFSDMKFSSTPKSINNIKSGEYERLDTGIMELNRVLG
GGIVKGSITLISGAPGIGKSTLLLQTASNIAEKYGKVLYVSGEESEEQIKMRGDRLDSLSNDLYIVSEVNIDKIKAHIEQ
TKPVFVVVDSIQTLFKESISSAPGSVSQVRETSNDIMRIGKSNNISFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
GELRILRTVKNRFGTTSEIGVFEMAGSGLKEILNPSAAFLEDREFQKEGSVIIGIMEGKRPILVEIQALVSETKAVMPRR
TAVGVDISRLNLILAVLEKKLRITFYNCDVYVNVVGGLKIEGTFGDLGLALALISSAKSREIGLDNLLAVGEIGLTSEVR
PVSFCDRIVNEAKKMGFRNIIIPDRNNGKVNCKDINVIGVSSLKEALNKVF

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=223581 B5S50_RS10070 WP_217303703.1 2177139..2178494(-) (radA) [Clostridium sp. 001]
ATGGTAAAGAAAAAAACGGTTTTTGTATGCCAGGAATGTGGATATGAATCCCCAAAGTGGCTGGGTAAATGTCCTAATTG
TAATAAATGGAATTGCATGGTAGAAGAGATTAAGTCGCCTCAGGAAGTAACATTTTCTGATATGAAGTTTAGCAGCACAC
CCAAGAGTATAAATAATATAAAATCCGGTGAATATGAAAGGTTAGATACTGGTATAATGGAATTGAATAGAGTTCTTGGA
GGAGGCATTGTAAAAGGTTCAATAACACTTATATCCGGAGCTCCTGGTATTGGAAAATCGACTTTGCTTTTACAGACGGC
CAGCAATATTGCTGAAAAGTATGGAAAAGTGCTGTATGTCTCAGGAGAAGAGTCAGAAGAACAGATAAAGATGAGGGGAG
ATAGACTTGATTCTTTGTCTAATGATCTTTATATAGTTTCAGAAGTTAATATTGATAAAATAAAGGCTCATATAGAACAA
ACTAAACCTGTATTTGTAGTAGTGGATTCTATTCAGACTTTATTTAAAGAATCTATTAGCTCAGCACCGGGTAGTGTGTC
TCAAGTAAGGGAAACTTCTAACGATATTATGCGTATAGGGAAGTCTAATAATATATCTTTTTTTATAGTGGCACATGTTA
CAAAGCAGGGTGAGCTAGCGGGACCTAGGGTACTAGAGCATATGGTGGATACTGTACTGTCTTTTGAAGGAGAAAGAACA
GGAGAGTTAAGAATTTTAAGAACTGTAAAAAACCGTTTTGGTACTACCAGTGAAATTGGTGTATTTGAAATGGCAGGAAG
CGGGCTTAAAGAAATATTAAATCCTTCTGCAGCATTTTTAGAAGATAGAGAATTTCAAAAAGAAGGTTCTGTAATTATTG
GTATAATGGAAGGAAAAAGGCCTATACTAGTAGAAATACAAGCGTTGGTTAGTGAAACTAAAGCAGTTATGCCAAGAAGA
ACAGCAGTTGGAGTAGATATCTCCAGATTAAATTTAATTTTAGCGGTACTTGAGAAAAAACTTAGAATTACATTTTATAA
TTGTGATGTTTATGTAAATGTAGTAGGAGGACTTAAGATAGAAGGAACTTTTGGAGATTTAGGGCTTGCCCTTGCACTTA
TATCTAGTGCTAAATCTAGGGAAATAGGTCTAGATAATCTTTTGGCAGTAGGTGAGATTGGACTTACAAGTGAAGTTAGA
CCAGTATCATTTTGTGATAGAATTGTAAATGAAGCAAAAAAAATGGGATTTAGGAATATAATAATACCAGATAGAAATAA
TGGAAAGGTAAATTGTAAAGATATAAATGTTATAGGTGTATCCTCCCTGAAGGAGGCATTAAATAAAGTTTTTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

AAA domain (AAA_25)

(61-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.993

100

0.512
  radA Streptococcus pneumoniae Rx1

48.904

100

0.494
  radA Streptococcus pneumoniae D39

48.904

100

0.494
  radA Streptococcus pneumoniae R6

48.904

100

0.494
  radA Streptococcus pneumoniae TIGR4

48.904

100

0.494
  radA Streptococcus mitis NCTC 12261

48.684

100

0.492
  radA Streptococcus mitis SK321

48.684

100

0.492

Multiple sequence alignment