Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   A6J74_RS24540 Genome accession   NZ_CP020437
Coordinates   4291024..4291458 (+) Length   144 a.a.
NCBI ID   WP_000811503.1    Uniprot ID   -
Organism   Bacillus sp. FDAARGOS_235     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4286024..4296458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J74_RS24515 (A6J74_24555) - 4286929..4288092 (-) 1164 WP_000434560.1 aminotransferase A -
  A6J74_RS24520 (A6J74_24560) - 4288211..4288411 (-) 201 WP_000929282.1 hypothetical protein -
  A6J74_RS24525 (A6J74_24565) - 4288723..4289997 (+) 1275 WP_000420366.1 HAMP domain-containing sensor histidine kinase -
  A6J74_RS24530 (A6J74_24570) - 4290036..4290785 (-) 750 WP_000387494.1 DUF3967 domain-containing protein -
  A6J74_RS24540 (A6J74_24580) nucA/comI 4291024..4291458 (+) 435 WP_000811503.1 DNA-entry nuclease Machinery gene
  A6J74_RS24545 (A6J74_24585) - 4291494..4291883 (+) 390 WP_000424018.1 competence protein ComJ -
  A6J74_RS24550 (A6J74_24590) metE 4291921..4294209 (-) 2289 WP_001007623.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  A6J74_RS24555 (A6J74_24595) - 4294733..4295290 (-) 558 WP_000062054.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=223078 A6J74_RS24540 WP_000811503.1 4291024..4291458(+) (nucA/comI) [Bacillus sp. FDAARGOS_235]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAGDRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=223078 A6J74_RS24540 WP_000811503.1 4291024..4291458(+) (nucA/comI) [Bacillus sp. FDAARGOS_235]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACGAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATTGATCGTGGTGGTGCTGGAGATAGAAGGAAA
TTATCGTTAGCTCCATATCCATCAAAAAAAGGCTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGGGCGCATATTGAATATATAAGTCCAGCGGATAATCGCGGAGCAGGTTCTTGGGTAGGAAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(50-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment