Detailed information NTG0022307

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PFL_RS26850
• Genome accession: NC_004129
• Coordinates: 6058937..6059809 (+)
• Length: 290 a.a.
• NCBI ID: WP_011063522.1
• Uniprot ID: Q4K5X2
• Organism: Pseudomonas protegens Pf-5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6053937..6064809

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PFL_RS26835 (PFL_5286)	-	6056386..6056742 (-)	357	WP_011063519.1	BON domain-containing protein	-
PFL_RS26840 (PFL_5287)	-	6056971..6057387 (-)	417	WP_011063520.1	pilin	-
PFL_RS26845 (PFL_5288)	pilC	6057717..6058934 (+)	1218	WP_011063521.1	type II secretion system F family protein	Machinery gene
PFL_RS26850 (PFL_5289)	pilD	6058937..6059809 (+)	873	WP_011063522.1	prepilin peptidase	Machinery gene
PFL_RS26855 (PFL_5290)	coaE	6059806..6060429 (+)	624	WP_011063523.1	dephospho-CoA kinase	-
PFL_RS26860 (PFL_5291)	yacG	6060426..6060629 (+)	204	WP_011063524.1	DNA gyrase inhibitor YacG	-
PFL_RS26865 (PFL_5292)	-	6060649..6060864 (-)	216	WP_011063525.1	hypothetical protein	-
PFL_RS26870 (PFL_5293)	-	6060928..6061617 (-)	690	WP_011063526.1	energy-coupling factor ABC transporter permease	-
PFL_RS26875 (PFL_5294)	-	6061614..6062081 (-)	468	WP_026019989.1	hypothetical protein	-
PFL_RS26880 (PFL_5295)	-	6062225..6062854 (+)	630	WP_011063528.1	DUF1780 domain-containing protein	-
PFL_RS26885	-	6063044..6063217 (+)	174	WP_015637073.1	DUF3094 family protein	-
PFL_RS26890 (PFL_5296)	-	6063278..6064576 (+)	1299	WP_011063529.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32053.26 Da
• Isoelectric Point: 7.0083

>NTDB_id=22307 PFL_RS26850 WP_011063522.1 6058937..6059809(+) (pilD) [Pseudomonas protegens Pf-5]
MPLTDALTLYPMAFVAAALVLGLIVGSFLNVLVWRLPKMLERDWRAQARDVLGLGPEPVAAPYNLMLPHSQCPHCAQAIR
PWENIPLLSYLWLRGCCAHCNSSISPRYPLTELACGLISGFIAWHFGFGWAAGLMLILSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANSFGLLISLEDALWGTVIGYLFLWSVFWLFKLLTGKEGMGHGDFKLLALFGAWGGWQILPLTLLLSSLLG
AILGTILLRLRREKTSTPIPFGPFLAIAGWIALLWGGQITDFYLQFVGFQ

Nucleotide

• Length: 873 bp

>NTDB_id=22307 PFL_RS26850 WP_011063522.1 6058937..6059809(+) (pilD) [Pseudomonas protegens Pf-5]
ATGCCCCTGACTGACGCCCTCACTCTCTACCCCATGGCCTTCGTGGCTGCGGCCCTGGTATTGGGCCTGATCGTCGGCAG
CTTTCTCAACGTGCTGGTGTGGCGCCTGCCGAAAATGCTCGAACGCGACTGGCGAGCCCAGGCCCGCGACGTGCTGGGGC
TGGGGCCGGAACCTGTGGCTGCGCCCTATAACCTGATGCTGCCGCACTCCCAGTGCCCGCACTGTGCGCAGGCGATCCGC
CCCTGGGAAAACATTCCGCTGCTCAGTTACCTGTGGCTGCGTGGCTGCTGCGCCCACTGCAATAGCTCCATCAGCCCGCG
CTACCCGTTGACCGAACTGGCCTGCGGGCTGATCTCGGGTTTTATCGCCTGGCACTTCGGCTTCGGCTGGGCGGCCGGGC
TGATGCTGATCCTCAGTTGGGGGCTGCTGGCAATGAGCCTGATCGACGCCGATCACCAGCTGCTGCCGGATGTGCTGGTG
CTGCCACTGTTGTGGCTGGGGCTGATCGCCAACAGCTTCGGCCTGCTGATCAGCCTGGAGGATGCGCTGTGGGGGACGGT
GATCGGCTACCTGTTCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGCTGACCGGCAAGGAGGGCATGGGCCACGGTGATT
TCAAGCTGCTGGCCCTGTTCGGGGCCTGGGGCGGCTGGCAGATCCTGCCTCTGACCCTGCTGCTGTCATCCCTGTTGGGA
GCGATTCTCGGCACGATCCTGCTGCGCCTGCGCCGGGAAAAGACCTCGACGCCAATCCCCTTTGGACCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTTCTATCTGCAGTTTGTCGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: Q4K5X2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.317	95.862	0.521
pilD	Acinetobacter baumannii D1279779	51.46	94.483	0.486
pilD	Acinetobacter nosocomialis M2	51.46	94.483	0.486
pilD	Vibrio campbellii strain DS40M4	49.645	97.241	0.483
pilD	Neisseria gonorrhoeae MS11	47.037	93.103	0.438