Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   C2I05_RS02765 Genome accession   NZ_CP026039
Coordinates   478376..478786 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain PK1_2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 473376..483786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2I05_RS02735 (C2I05_02715) - 473462..473602 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  C2I05_RS02740 (C2I05_02720) yqeF 473908..474639 (-) 732 WP_249848127.1 SGNH/GDSL hydrolase family protein -
  C2I05_RS02745 (C2I05_02725) cwlH 474891..475643 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  C2I05_RS02750 (C2I05_02730) yqeD 475830..476456 (+) 627 WP_021480068.1 TVP38/TMEM64 family protein -
  C2I05_RS02755 (C2I05_02735) gnd 476476..477369 (-) 894 WP_249848124.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  C2I05_RS02760 (C2I05_02740) yqeB 477621..478343 (+) 723 WP_049141193.1 hypothetical protein -
  C2I05_RS02765 (C2I05_02745) nucA/comI 478376..478786 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  C2I05_RS02770 (C2I05_02750) - 478982..479332 (+) 351 Protein_552 sigma-70 family RNA polymerase sigma factor -
  C2I05_RS02775 (C2I05_02755) spoIVCA 479360..480820 (-) 1461 WP_249848478.1 site-specific DNA recombinase SpoIVCA -
  C2I05_RS02780 (C2I05_02760) - 480778..480956 (-) 179 Protein_554 hypothetical protein -
  C2I05_RS02785 (C2I05_02765) - 481476..482456 (-) 981 WP_249848122.1 DUF5677 domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=222912 C2I05_RS02765 WP_009967785.1 478376..478786(-) (nucA/comI) [Bacillus subtilis strain PK1_2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=222912 C2I05_RS02765 WP_009967785.1 478376..478786(-) (nucA/comI) [Bacillus subtilis strain PK1_2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529