Detailed information NTG0022263

Bioinformatically predicted

Overview

• Name: clpE
• Type: Regulator
• Locus tag: SAG_RS05270
• Genome accession: NC_004116
• Coordinates: 501870..504131 (-)
• Length: 753 a.a.
• NCBI ID: WP_000882549.1
• Uniprot ID: A0A0E1EFR4
• Organism: Streptococcus agalactiae 2603V/R
• Function: repress competence development (at the early growth phase) (predicted from homology) Competence regulation

Genomic Context

Location: 496870..509131

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAG_RS05250 (SAG0484)	-	496899..497669 (+)	771	WP_001131467.1	DivIVA domain-containing protein	-
SAG_RS05255 (SAG0485)	ileS	497954..500746 (+)	2793	WP_000768163.1	isoleucine--tRNA ligase	-
SAG_RS05260 (SAG0486)	-	500863..501165 (-)	303	WP_001237035.1	DUF1827 family protein	-
SAG_RS05265 (SAG0487)	-	501229..501684 (-)	456	WP_000184292.1	NUDIX hydrolase	-
SAG_RS05270 (SAG0488)	clpE	501870..504131 (-)	2262	WP_000882549.1	ATP-dependent Clp protease ATP-binding subunit	Regulator
SAG_RS05275 (SAG0490)	-	504429..504659 (+)	231	WP_000443582.1	DUF1797 family protein	-
SAG_RS05280 (SAG0491)	-	504801..505493 (+)	693	WP_001011647.1	amino acid ABC transporter permease	-
SAG_RS05285 (SAG0492)	-	505486..506220 (+)	735	WP_000230134.1	amino acid ABC transporter ATP-binding protein	-
SAG_RS05290 (SAG0493)	-	506507..508201 (+)	1695	WP_001106845.1	phospho-sugar mutase	-

Sequence

Protein

• Length: 753 a.a.
• Molecular weight: 83766.92 Da
• Isoelectric Point: 5.2771

>NTDB_id=22263 SAG_RS05270 WP_000882549.1 501870..504131(-) (clpE) [Streptococcus agalactiae 2603V/R]
MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPLFSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDL
PNTPPTQSGGNRGGGNGNGRNNNRNQTATPSQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRQRQDVILFIDEIHEIVG
AGTAGEGSMDAGNILKPALARGELQLVGATTLNEYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKY
NNDAIEAAAVLSNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAAYFRDQIAK
YKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLADDLKQHVIGQDDAVVKIAKAIRRNRVGLGSP
NRPIGSFLFVGPTGVGKTELSKQLAIELFGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLI
LLDEIEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGRTNSVLGQLGNFFSPEF
MNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDVTDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYY
LENPSEKELRAIMTSNGNIIIKSSKKTEESTKG

Nucleotide

• Length: 2262 bp

>NTDB_id=22263 SAG_RS05270 WP_000882549.1 501870..504131(-) (clpE) [Streptococcus agalactiae 2603V/R]
ATGCTCTGTCAAAACTGCAAACTAAACGAATCAACTATTCATTTGTATACAAATGTTAATGGAAAACAAAAGCAAGTTGA
TCTTTGTCAAAATTGCTACCAAATTATTAAAACTGACCCTAATAATCCACTTTTTTCAGGTTTAAATCATGTTTCACATG
CGCCTGGTGGCATCAATCCTTTCTTTGATGATTTCTTTGGAGATTTAAATAATTTCAGAGCCTTTAATGGCCAAGATTTA
CCAAACACTCCTCCTACACAATCAGGAGGAAACCGAGGTGGAGGAAACGGTAATGGACGAAATAATAATCGCAACCAAAC
TGCAACTCCTTCTCAAGCCAAAGGGATTCTAGAAGAATTTGGTATCAATGTCACTGAAATAGCCCGCCATGGTGATATTG
ACCCTGTTATTGGACGTGACTCAGAAATTATTCGTGTTATCGAAATCCTTAATCGTCGTACAAAAAATAATCCTGTACTT
ATTGGCGAACCCGGCGTTGGGAAAACTGCCGTTGTTGAAGGACTTGCTCAGAAAATTGTTGATGGTAATGTGCCTCATAA
ACTTCAAGGCAAACAAGTTATTCGTTTGGATGTTGTGAGTCTAGTACAAGGAACGGGCATTCGTGGTCAATTTGAAGAAC
GCATGCAAAAGTTGATGGAAGAAATTCGTCAACGTCAAGATGTTATTCTATTCATAGATGAAATTCACGAAATAGTTGGA
GCTGGTACAGCTGGCGAAGGTAGTATGGATGCAGGTAATATTTTAAAACCTGCCCTTGCACGTGGAGAATTACAACTAGT
AGGTGCTACTACTCTCAATGAATATCGTATTATCGAAAAGGACGCTGCCTTAGAACGCCGTATGCAACCTGTAAAAGTCG
ATGAGCCTTCTGTTGAAGAAACCATTACTATTTTGAAAGGTATCCAAAAAAAATACGAAGATTATCATCATGTAAAATAT
AATAATGATGCCATAGAAGCAGCTGCAGTACTATCTAATCGTTATATCCAGGACCGCTTTTTACCTGATAAAGCAATAGA
CTTATTAGATGAAGCTGGTTCTAAAATGAACCTAACACTAAATTTTGTTGATCCAAAAGAAATTGATCAACGTCTCATTG
AAGCAGAAAATTTAAAAGCGCAAGCGACTCGTGAAGAAGATTACGAACGTGCAGCTTACTTCCGTGACCAGATTGCAAAA
TATAAAGAAATGCAACAACAAAAGGTCGACGATCAAGATACACCTATTATTACCGAAAAAACAATTGAGCACATCATTGA
AGAAAAAACGAATATCCCTGTTGGTGATTTAAAAGAAAAAGAACAATCTCAATTAATTAATCTCGCAGATGACTTGAAAC
AACATGTGATCGGCCAGGATGACGCTGTCGTTAAGATTGCAAAAGCTATTCGTCGTAATCGAGTTGGTCTTGGTAGCCCA
AACCGTCCTATTGGTTCCTTTTTATTTGTAGGACCAACCGGCGTTGGTAAAACTGAACTTTCTAAACAACTAGCAATTGA
GCTCTTTGGTTCAGCTGATAGTATGATTCGTTTTGATATGTCAGAGTACATGGAAAAGCATGCTGTTGCTAAATTAGTCG
GAGCGCCTCCAGGATACGTGGGATACGAGGAAGCTGGACAATTAACTGAAAAGGTTCGTCGAAATCCTTACTCGCTCATC
CTTCTAGATGAAATTGAAAAAGCTCATCCTGATGTCATGCATATGTTCTTGCAGGTCCTTGATGACGGTCGATTAACAGA
TGGACAAGGAAGAACTGTTAGTTTTAAAGATACCATTATCATCATGACCTCAAATGCTGGTTCTGGTAAAACTGAAGCAA
GCGTTGGCTTTGGTGCCTCACGAGAAGGTAGAACGAATTCCGTACTAGGTCAACTAGGTAACTTCTTCAGCCCTGAATTT
ATGAACCGCTTTGACGGTATTATTGAATTCAAGGCTTTAGATAAAGAGAATCTCCTCAATATCGTTGATATTATGTTATC
TGACGTTAATGCACGTCTCGCCATCAATGGTATTCATCTAGATGTCACTGATAAAGTGAAAGAAAAATTGGTTGATTTAG
GTTACGATCCCAAAATGGGAGCACGTCCATTACGTCGTACCATTCAAGAACATATTGAAGATGCTATCACAGATTACTAT
CTTGAGAATCCAAGCGAAAAAGAACTTCGTGCTATTATGACTAGCAATGGAAATATCATAATAAAATCTTCTAAAAAAAC
TGAAGAAAGTACAAAAGGTTGA

Domains

Predicted by InterproScan.

• AAA domain (Cdc48 subfamily) (AAA_2) (482-645)

• ATPase family associated with various cellular activities (AAA) (AAA) (159-276)

• C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small) (653-727)

• AAA lid domain (AAA_lid_9) (297-394)

3D structure

• AlphaFold DB: A0A0E1EFR4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
clpE	Streptococcus mutans UA159	85.676	100	0.858
clpE	Streptococcus pneumoniae Rx1	85.714	99.469	0.853
clpE	Streptococcus pneumoniae D39	85.714	99.469	0.853
clpE	Streptococcus pneumoniae R6	85.714	99.469	0.853
clpE	Streptococcus pneumoniae TIGR4	85.581	99.469	0.851
clpC	Lactococcus lactis subsp. cremoris KW2	74.867	99.867	0.748
clpC	Bacillus subtilis subsp. subtilis str. 168	51.327	90.04	0.462
clpC	Lactococcus lactis subsp. lactis strain DGCC12653	47.528	83.267	0.396
clpC	Streptococcus pneumoniae Rx1	47.003	84.197	0.396
clpC	Streptococcus pneumoniae D39	47.003	84.197	0.396
clpC	Streptococcus pneumoniae TIGR4	46.845	84.197	0.394
clpC	Streptococcus thermophilus LMD-9	46.276	83.798	0.388
clpC	Streptococcus mutans UA159	46.87	82.736	0.388
clpC	Streptococcus thermophilus LMG 18311	45.959	83.798	0.385