Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BAJT_RS12135 Genome accession   NZ_CP020375
Coordinates   2519012..2519626 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain JTYP2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2514012..2524626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAJT_RS12110 (BAJT_12110) rpsT 2514315..2514581 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  BAJT_RS12115 (BAJT_12115) holA 2514598..2515639 (-) 1042 Protein_2340 DNA polymerase III subunit delta -
  BAJT_RS19595 - 2515679..2515830 (-) 152 Protein_2341 hypothetical protein -
  BAJT_RS12120 (BAJT_12120) - 2515871..2516005 (+) 135 WP_003152870.1 YqzM family protein -
  BAJT_RS12125 (BAJT_12125) comEC 2516024..2518375 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BAJT_RS12130 (BAJT_12130) - 2518376..2518945 (-) 570 WP_003152868.1 ComE operon protein 2 -
  BAJT_RS12135 (BAJT_12135) comEA 2519012..2519626 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  BAJT_RS12140 (BAJT_12140) comER 2519685..2520506 (+) 822 WP_012118027.1 late competence protein ComER -
  BAJT_RS12145 (BAJT_12145) - 2520575..2521312 (-) 738 WP_032873951.1 class I SAM-dependent DNA methyltransferase -
  BAJT_RS12150 (BAJT_12150) rsfS 2521309..2521665 (-) 357 WP_007408260.1 ribosome silencing factor -
  BAJT_RS12155 (BAJT_12155) yqeK 2521669..2522244 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BAJT_RS12160 (BAJT_12160) - 2522234..2522803 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  BAJT_RS12165 (BAJT_12165) yhbY 2522814..2523104 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  BAJT_RS12170 (BAJT_12170) aroE 2523098..2523940 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=221918 BAJT_RS12135 WP_032873955.1 2519012..2519626(-) (comEA) [Bacillus velezensis strain JTYP2]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=221918 BAJT_RS12135 WP_032873955.1 2519012..2519626(-) (comEA) [Bacillus velezensis strain JTYP2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363

Multiple sequence alignment