Detailed information NTG0022188

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: SPYM3_RS04445
• Genome accession: NC_004070
• Coordinates: 871597..872433 (+)
• Length: 278 a.a.
• NCBI ID: WP_011054501.1
• Uniprot ID: A0A5S4TKN4
• Organism: Streptococcus pyogenes MGAS315
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 866597..877433

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYM3_RS04415 (SpyM3_0813)	-	867145..867519 (+)	375	WP_011054498.1	hypothetical protein	-
SPYM3_RS04420 (SpyM3_0814)	-	867569..868480 (-)	912	WP_002984631.1	diacylglycerol/lipid kinase family protein	-
SPYM3_RS04425 (SpyM3_0815)	trhA	868597..869247 (-)	651	WP_002984629.1	PAQR family membrane homeostasis protein TrhA	-
SPYM3_RS04430 (SpyM3_0816)	-	869244..869684 (-)	441	WP_002989774.1	DUF1836 domain-containing protein	-
SPYM3_RS04435 (SpyM3_0817)	ylqF	869903..870751 (+)	849	WP_011054499.1	ribosome biogenesis GTPase YlqF	-
SPYM3_RS04440 (SpyM3_0818)	-	870741..871532 (+)	792	WP_011054500.1	ribonuclease HII	-
SPYM3_RS04445 (SpyM3_0819)	dprA	871597..872433 (+)	837	WP_011054501.1	DNA-processing protein DprA	Machinery gene
SPYM3_RS04450 (SpyM3_0820)	topA	872539..874668 (+)	2130	WP_011054502.1	type I DNA topoisomerase	-
SPYM3_RS04455 (SpyM3_0821)	trmFO	874803..876149 (+)	1347	WP_032461335.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-

Sequence

Protein

• Length: 278 a.a.
• Molecular weight: 31087.14 Da
• Isoelectric Point: 9.4248

>NTDB_id=22188 SPYM3_RS04445 WP_011054501.1 871597..872433(+) (dprA) [Streptococcus pyogenes MGAS315]
MNHFELYKLKKAGLTNKNILNILDYQKHQEKSLSLRDMAVVSGCKHPSHFIEAYKQLDIQKLKMEFKQFPSISILDKHYP
MALKEIYNPPVLLFFQGNLDLLEKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPREEALSYHFPERNRIIAGLSRGILVVEAKNRSGSLITCQIGIEEGR
DIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILSEY

Nucleotide

• Length: 837 bp

>NTDB_id=22188 SPYM3_RS04445 WP_011054501.1 871597..872433(+) (dprA) [Streptococcus pyogenes MGAS315]
GTGAATCATTTTGAACTTTATAAGCTAAAGAAAGCTGGACTGACTAATAAAAACATTCTCAATATTCTTGACTACCAAAA
ACACCAGGAAAAATCGTTGTCACTTCGAGATATGGCCGTTGTTTCTGGTTGTAAGCATCCGTCTCACTTTATAGAAGCCT
ATAAGCAGTTAGATATTCAAAAATTAAAAATGGAATTTAAACAATTTCCTAGTATTTCTATCTTAGATAAGCATTACCCA
ATGGCATTGAAAGAAATATACAATCCACCTGTCCTCTTGTTTTTTCAGGGAAATTTAGACCTTCTTGAGAAACCTAAATT
AGCCATTGTCGGCTCCAGACGCTCAAGCGATACCGGAGTAAAGTCTGTCCGTAAAATTCTTAAAGAACTCGGGAACCGTT
TTGTGATTGTTAGCGGACTTGCTCGAGGTATCGACACTAGTGCTCATTTAGCCTGCCTTAAAAATGGGGGACAAACCATC
GCTATTATTGGAACAGGGTTGGATCGCTTTTACCCTAAAGAAAATAGGGAGTTGCAAACTTTCTTAGGGAAAAATCATCT
TGTGCTAACAGAATACGGTCCAAGAGAAGAAGCTTTATCTTATCACTTTCCAGAACGGAATCGTATTATCGCAGGCCTTA
GCCGAGGTATTCTTGTCGTTGAAGCAAAAAATCGTTCAGGTTCCTTGATTACTTGTCAAATTGGGATAGAAGAAGGCCGA
GACATTTTTGCTGTCCCAGGAAACATTTTGGACGGGAAATCCGAAGGTTGCCTTCAGTTAATTAAAGAGGGAGCAACATG
CGTCACATCGGGCATGGATATCCTTTCAGAGTACTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

• DNA recombination-mediator protein A (DNA_processg_A) (68-269)

3D structure

• AlphaFold DB: A0A5S4TKN4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	67.266	100	0.673
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae D39	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae R6	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	60.072	100	0.601
dprA/cilB/dalA	Streptococcus mitis SK321	60.072	100	0.601
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	59.712	100	0.597
dprA	Lactococcus lactis subsp. cremoris KW2	57.194	100	0.572
dprA	Legionella pneumophila strain ERS1305867	42.857	85.612	0.367