Detailed information NTG0221864

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: C2H82_RS14890
• Genome accession: NZ_CP026027
• Coordinates: 2849874..2850866 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081588.1
• Uniprot ID: Q540P0
• Organism: Escherichia coli strain LIM
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2844874..2855866

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C2H82_RS14870	otnK	2845650..2846816 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
C2H82_RS14875	ygbL	2846908..2847546 (+)	639	WP_001278994.1	aldolase	-
C2H82_RS14880	ygbM	2847551..2848327 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
C2H82_RS14885	ygbN	2848416..2849780 (+)	1365	WP_000104441.1	GntP family transporter	-
C2H82_RS14890	rpoS	2849874..2850866 (-)	993	WP_000081588.1	RNA polymerase sigma factor RpoS	Regulator
C2H82_RS14895	nlpD	2850929..2852023 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
C2H82_RS14900	pcm	2852208..2852834 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
C2H82_RS14905	surE	2852828..2853589 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
C2H82_RS14910	truD	2853570..2854619 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
C2H82_RS14915	ispF	2854616..2855095 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
C2H82_RS14920	ispD	2855095..2855805 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37971.86 Da
• Isoelectric Point: 4.5886

>NTDB_id=221864 C2H82_RS14890 WP_000081588.1 2849874..2850866(-) (rpoS) [Escherichia coli strain LIM]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=221864 C2H82_RS14890 WP_000081588.1 2849874..2850866(-) (rpoS) [Escherichia coli strain LIM]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

3D structure

• AlphaFold DB: Q540P0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715