Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | BSK2_RS12950 | Genome accession | NZ_CP020367 |
| Coordinates | 2528293..2528703 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain GQJK2 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2523659..2547126 | 2528293..2528703 | within | 0 |
Gene organization within MGE regions
Location: 2523659..2547126
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BSK2_RS12925 (BSK2_12925) | yqeF | 2523826..2524557 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| BSK2_RS12930 (BSK2_12930) | cwlH | 2524809..2525561 (-) | 753 | WP_080529562.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| BSK2_RS12935 (BSK2_12935) | yqeD | 2525748..2526374 (+) | 627 | WP_080529563.1 | TVP38/TMEM64 family protein | - |
| BSK2_RS12940 (BSK2_12940) | gnd | 2526393..2527286 (-) | 894 | WP_038429331.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| BSK2_RS12945 (BSK2_12945) | yqeB | 2527538..2528260 (+) | 723 | WP_080529564.1 | hypothetical protein | - |
| BSK2_RS12950 (BSK2_12950) | nucA/comI | 2528293..2528703 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| BSK2_RS12955 (BSK2_12955) | - | 2528899..2529249 (+) | 351 | Protein_2511 | sigma-70 family RNA polymerase sigma factor | - |
| BSK2_RS12960 (BSK2_12960) | - | 2529389..2529859 (+) | 471 | WP_080529565.1 | MarR family transcriptional regulator | - |
| BSK2_RS12965 (BSK2_12965) | - | 2530011..2531423 (+) | 1413 | WP_080529566.1 | MDR family MFS transporter | - |
| BSK2_RS12970 (BSK2_12970) | spoIVCA | 2531610..2533070 (-) | 1461 | WP_250622053.1 | site-specific DNA recombinase SpoIVCA | - |
| BSK2_RS21440 (BSK2_12975) | - | 2533028..2533206 (-) | 179 | Protein_2515 | hypothetical protein | - |
| BSK2_RS12980 (BSK2_12980) | - | 2533873..2534970 (-) | 1098 | WP_080529568.1 | glycosyl hydrolase | - |
| BSK2_RS12990 (BSK2_12990) | - | 2535504..2536142 (-) | 639 | WP_080529569.1 | HD domain-containing protein | - |
| BSK2_RS12995 (BSK2_12995) | - | 2536227..2536850 (-) | 624 | WP_080529570.1 | FMN-binding negative transcriptional regulator | - |
| BSK2_RS13000 (BSK2_13000) | - | 2536876..2537394 (-) | 519 | WP_080529571.1 | GNAT family N-acetyltransferase | - |
| BSK2_RS13005 (BSK2_13005) | - | 2537413..2537868 (-) | 456 | WP_080529572.1 | MarR family transcriptional regulator | - |
| BSK2_RS13010 (BSK2_13010) | - | 2537983..2538630 (+) | 648 | WP_080529573.1 | YitT family protein | - |
| BSK2_RS13015 (BSK2_13015) | - | 2538737..2539537 (-) | 801 | WP_080529574.1 | dihydrodipicolinate synthase family protein | - |
| BSK2_RS13020 (BSK2_13020) | - | 2539687..2541066 (+) | 1380 | WP_080529575.1 | PLP-dependent aminotransferase family protein | - |
| BSK2_RS13030 (BSK2_13030) | - | 2541584..2542714 (-) | 1131 | WP_032726212.1 | hypothetical protein | - |
| BSK2_RS13035 (BSK2_13035) | - | 2542900..2544780 (+) | 1881 | WP_080529576.1 | T7SS effector LXG polymorphic toxin | - |
| BSK2_RS13040 (BSK2_13040) | - | 2544785..2545084 (+) | 300 | WP_080529577.1 | contact-dependent growth inhibition system immunity protein | - |
| BSK2_RS13045 (BSK2_13045) | cwlA | 2545123..2545941 (-) | 819 | WP_080529578.1 | N-acetylmuramoyl-L-alanine amidase | - |
| BSK2_RS13050 (BSK2_13050) | - | 2545986..2546126 (-) | 141 | Protein_2528 | phage holin family protein | - |
| BSK2_RS13055 (BSK2_13055) | - | 2546153..2546338 (-) | 186 | Protein_2529 | phage terminase large subunit | - |
| BSK2_RS13060 (BSK2_13060) | terS | 2546335..2547126 (-) | 792 | WP_080529579.1 | phage terminase small subunit | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=221770 BSK2_RS12950 WP_009967785.1 2528293..2528703(-) (nucA/comI) [Bacillus subtilis strain GQJK2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=221770 BSK2_RS12950 WP_009967785.1 2528293..2528703(-) (nucA/comI) [Bacillus subtilis strain GQJK2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |