Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BSK2_RS12705 Genome accession   NZ_CP020367
Coordinates   2484853..2485620 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain GQJK2     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2479853..2490620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSK2_RS12685 (BSK2_12685) ppsR 2480028..2480840 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  BSK2_RS12690 (BSK2_12690) ccpN 2480866..2481504 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  BSK2_RS12695 (BSK2_12695) glyS 2481637..2483676 (-) 2040 WP_024572413.1 glycine--tRNA ligase subunit beta -
  BSK2_RS12700 (BSK2_12700) glyQ 2483669..2484556 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  BSK2_RS12705 (BSK2_12705) recO 2484853..2485620 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  BSK2_RS12710 (BSK2_12710) yqzL 2485657..2485800 (-) 144 WP_003230053.1 YqzL family protein -
  BSK2_RS12715 (BSK2_12715) era 2485948..2486853 (-) 906 WP_003230051.1 GTPase Era -
  BSK2_RS12720 (BSK2_12720) - 2486834..2487244 (-) 411 WP_003230049.1 cytidine deaminase -
  BSK2_RS12725 (BSK2_12725) unpK 2487363..2487734 (-) 372 WP_080529555.1 diacylglycerol kinase family protein -
  BSK2_RS12730 (BSK2_12730) ybeY 2487715..2488188 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  BSK2_RS12735 (BSK2_12735) pgpH 2488189..2490324 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=221766 BSK2_RS12705 WP_014477369.1 2484853..2485620(-) (recO) [Bacillus subtilis strain GQJK2]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=221766 BSK2_RS12705 WP_014477369.1 2484853..2485620(-) (recO) [Bacillus subtilis strain GQJK2]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996

Multiple sequence alignment