Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BSK2_RS01960 Genome accession   NZ_CP020367
Coordinates   364789..365238 (-) Length   149 a.a.
NCBI ID   WP_033883649.1    Uniprot ID   -
Organism   Bacillus subtilis strain GQJK2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 359789..370238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSK2_RS20970 - 360229..360425 (+) 197 Protein_333 hypothetical protein -
  BSK2_RS01925 (BSK2_01925) - 360461..360781 (+) 321 Protein_334 RDD family protein -
  BSK2_RS01930 (BSK2_01930) yckD 360863..361195 (+) 333 WP_003246247.1 YckD family protein -
  BSK2_RS01935 (BSK2_01935) - 361323..361745 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  BSK2_RS01940 (BSK2_01940) - 361733..362074 (+) 342 WP_080528906.1 DUF3147 family protein -
  BSK2_RS01945 (BSK2_01945) - 362093..362821 (+) 729 WP_021481826.1 AAA family ATPase -
  BSK2_RS01950 (BSK2_01950) bglC 362894..364327 (+) 1434 WP_080528907.1 6-phospho-beta-glucosidase -
  BSK2_RS01955 (BSK2_01955) nin/comJ 364364..364762 (-) 399 WP_080528908.1 DNA-entry nuclease inhibitor Regulator
  BSK2_RS01960 (BSK2_01960) nucA/comI 364789..365238 (-) 450 WP_033883649.1 DNA-entry nuclease Machinery gene
  BSK2_RS01965 (BSK2_01965) tlpC 365406..367127 (-) 1722 WP_080528909.1 methyl-accepting chemotaxis protein TlpC -
  BSK2_RS01970 (BSK2_01970) hxlB 367238..367795 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  BSK2_RS01975 (BSK2_01975) hxlA 367801..368433 (-) 633 WP_080528910.1 3-hexulose-6-phosphate synthase -
  BSK2_RS01980 (BSK2_01980) hxlR 368665..369027 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16454.42 Da        Isoelectric Point: 4.5282

>NTDB_id=221725 BSK2_RS01960 WP_033883649.1 364789..365238(-) (nucA/comI) [Bacillus subtilis strain GQJK2]
MNITTDIIKTILLVIVIIAAAAIGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=221725 BSK2_RS01960 WP_033883649.1 364789..365238(-) (nucA/comI) [Bacillus subtilis strain GQJK2]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTATCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment