Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BLMD_RS13525 Genome accession   NZ_CP020352
Coordinates   2702870..2703934 (-) Length   354 a.a.
NCBI ID   WP_080131442.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain MDJK30     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2697870..2708934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLMD_RS13480 (BLMD_13480) tapA 2698364..2699092 (-) 729 WP_023855187.1 amyloid fiber anchoring/assembly protein TapA -
  BLMD_RS13485 (BLMD_13485) - 2699370..2699690 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  BLMD_RS13490 (BLMD_13490) - 2699720..2699902 (-) 183 WP_020452171.1 YqzE family protein -
  BLMD_RS13495 (BLMD_13495) comGG 2699991..2700356 (-) 366 WP_048349968.1 competence type IV pilus minor pilin ComGG -
  BLMD_RS13500 (BLMD_13500) comGF 2700368..2700850 (-) 483 WP_230459149.1 competence type IV pilus minor pilin ComGF -
  BLMD_RS13505 (BLMD_13505) comGE 2700765..2701112 (-) 348 WP_025811161.1 competence type IV pilus minor pilin ComGE -
  BLMD_RS13510 (BLMD_13510) comGD 2701096..2701533 (-) 438 WP_026579312.1 competence type IV pilus minor pilin ComGD -
  BLMD_RS13515 (BLMD_13515) comGC 2701539..2701832 (-) 294 WP_026579311.1 competence type IV pilus major pilin ComGC Machinery gene
  BLMD_RS13520 (BLMD_13520) comGB 2701846..2702880 (-) 1035 WP_026579310.1 competence type IV pilus assembly protein ComGB Machinery gene
  BLMD_RS13525 (BLMD_13525) comGA 2702870..2703934 (-) 1065 WP_080131442.1 competence type IV pilus ATPase ComGA Machinery gene
  BLMD_RS13530 (BLMD_13530) - 2704092..2704931 (-) 840 WP_035338373.1 STAS domain-containing protein -
  BLMD_RS13540 (BLMD_13540) - 2705173..2705550 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BLMD_RS13545 (BLMD_13545) - 2705761..2706006 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  BLMD_RS13550 (BLMD_13550) - 2706042..2706680 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  BLMD_RS13555 (BLMD_13555) - 2706836..2707009 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  BLMD_RS13560 (BLMD_13560) - 2707070..2707384 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -
  BLMD_RS13565 (BLMD_13565) - 2707400..2708509 (-) 1110 WP_048349964.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39796.34 Da        Isoelectric Point: 9.4163

>NTDB_id=221645 BLMD_RS13525 WP_080131442.1 2702870..2703934(-) (comGA) [Bacillus paralicheniformis strain MDJK30]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDRLTRNECSRLISHFKFLSSMDIGKRRLPQSGALT
LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLILFTGPTGSGKTTTLYSLIEY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLEFGVDMTEIEQTLVAISAQRLVGLVCPFCGDSCSLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GDIKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=221645 BLMD_RS13525 WP_080131442.1 2702870..2703934(-) (comGA) [Bacillus paralicheniformis strain MDJK30]
TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTCCGCTTCAGGATTGATGATGAACTTTTTCAAAAAGACAGATTAACGAGAAATGAGTGCT
CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGATATTGGGAAAAGACGGCTGCCGCAAAGCGGAGCTTTAACT
CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATTCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACACTCTATTCTTTAATTGAATAC
GCTAAGCGGCATTTTAAGCGAAATATCATTACCTTGGAAGACCCTGTTGAATCAAGAAGCGAAAACATTTTGCAAGTGCA
AGTAAACGAAAAAGCTGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTAG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT
GCGAAAAATGCCAAAGGCGCAATATACAGGCTGCTCGAGTTCGGCGTTGATATGACAGAAATTGAACAAACACTGGTCGC
AATCAGCGCCCAGCGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAGCTGCTCTTTATATTGCAGATTGTCGAGGC
CTGTGAGAAGAGCAAGCGTCTTCGAGCTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC
GGTGACATAAAAACAGAAACTTTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.514

100

0.675

Multiple sequence alignment