Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B5S52_RS19405 Genome accession   NZ_CP020350
Coordinates   4335638..4337020 (+) Length   460 a.a.
NCBI ID   WP_014914021.1    Uniprot ID   A0A3S0ZXC5
Organism   Pectobacterium brasiliense strain SX309     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4330638..4342020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5S52_RS19385 (B5S52_19385) prfC 4330941..4332530 (-) 1590 WP_039510188.1 peptide chain release factor 3 -
  B5S52_RS19390 (B5S52_19390) - 4332822..4333739 (+) 918 WP_039555515.1 hypothetical protein -
  B5S52_RS19395 (B5S52_19395) - 4333788..4334486 (-) 699 WP_039555514.1 YtjB family periplasmic protein -
  B5S52_RS19400 (B5S52_19400) serB 4334643..4335620 (+) 978 WP_039510182.1 phosphoserine phosphatase -
  B5S52_RS19405 (B5S52_19405) radA 4335638..4337020 (+) 1383 WP_014914021.1 DNA repair protein RadA Machinery gene
  B5S52_RS19410 (B5S52_19410) nadR 4337139..4338392 (+) 1254 WP_039279404.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  B5S52_RS19415 (B5S52_19415) - 4338389..4339036 (-) 648 WP_080164080.1 5-formyltetrahydrofolate cyclo-ligase -
  B5S52_RS19425 (B5S52_19425) zapA 4339334..4339663 (-) 330 WP_010284667.1 cell division protein ZapA -
  B5S52_RS19430 (B5S52_19430) - 4339856..4340443 (+) 588 WP_010284662.1 YecA family protein -
  B5S52_RS19435 (B5S52_19435) pepP 4340501..4341826 (+) 1326 WP_080164082.1 Xaa-Pro aminopeptidase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49620.19 Da        Isoelectric Point: 7.4218

>NTDB_id=221569 B5S52_RS19405 WP_014914021.1 4335638..4337020(+) (radA) [Pectobacterium brasiliense strain SX309]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASTSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKVPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=221569 B5S52_RS19405 WP_014914021.1 4335638..4337020(+) (radA) [Pectobacterium brasiliense strain SX309]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGACTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAATACCATTACCGAAGTCCGTCTGGCCTCGGCGTCAACATCACGTTCCGACCGTCTCACCGGCTATG
CGGGCGAAAGCGCTGGCGTCAGCCGGGTACAAAAGCTCTCGGAAATCAGTCTTGAAGCCCTGCCGCGCTTTTCCACCGGT
TTTCAGGAGTTTGACCGCGTTCTGGGCGGCGGCGTCGTTCCGGGCAGCGCGATTCTGATCGGCGGTAACCCTGGCGCGGG
TAAAAGTACCTTACTGCTGCAAACGCTCTGCAAGCTGTCAGAGAACATGAAAACCCTGTACGTCACCGGTGAAGAATCCT
TGCAGCAGGTGGCGATGCGGGCGCATCGTCTCAACCTGCCGACCCAAAATCTCAATATGCTGTCGGAAACCAGCATCGAA
CAGATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGCTGATGGTGATCGACTCCATTCAGGTGATGCATCTCGCTGATAT
TCAATCGTCACCCGGTAGCGTAGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTCGCCAAAACACGCGGCGTCG
CCATCGTGATGGTCGGCCACGTCACCAAAGACGGTTCGCTCGCCGGGCCAAAAGTGTTGGAACACTGCATCGACTGCTCC
GTGCTGCTGGATGGCGATGCCGATTCCCGCTTCCGCACCCTGCGCAGTCATAAAAACCGTTTCGGTGCCGTTAACGAGCT
GGGCGTATTCGCGATGACGGAACAGGGTCTACGCGAGATCAGCAATCCGTCGGCAATTTTCCTCAGTCGCGGTGACGAAA
TCACGTCCGGCAGTTCGGTCATGGTGGTGTGGGAAGGTACGCGTCCGCTGCTGGTCGAGATTCAGGCGCTGGTGGATCAA
TCGATGATGGCCAACCCGCGTCGCGTGGCGGTCGGGCTGGAGCAAAATCGCCTGGCGATCCTGCTGGCGGTATTACATCG
CCATGGCGGCTTGCAGATGTCCGATCAGGATGTGTTCGTGAATGTCGTCGGCGGCGTTAAAGTCACCGAAACCAGCGCCG
ACCTGGCGCTGCTGCTCTCGCTGGTCTCCAGCTTCCGCGACCGCCCACTGCCGCAGGATCTTGTCATCTTCGGTGAAGTC
GGTCTGGCGGGCGAAATCCGCCCGGTTCCGAGCGGCCAAGAGCGGATTACCGAAGCCGCCAAGCACGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAGTCCCTGCCAGCATGCAGGTGTTCGGCGTGAAAAAGCTGGCCGATGCGC
TGGCGATCCTCGACGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0ZXC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.261

100

0.483
  radA Streptococcus pneumoniae Rx1

42.982

99.13

0.426
  radA Streptococcus pneumoniae D39

42.982

99.13

0.426
  radA Streptococcus pneumoniae R6

42.982

99.13

0.426
  radA Streptococcus pneumoniae TIGR4

42.982

99.13

0.426
  radA Streptococcus mitis NCTC 12261

42.982

99.13

0.426
  radA Streptococcus mitis SK321

42.982

99.13

0.426

Multiple sequence alignment