Detailed information NTG0221562

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B5S52_RS07225
• Genome accession: NZ_CP020350
• Coordinates: 1585992..1586837 (+)
• Length: 281 a.a.
• NCBI ID: WP_040038049.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain SX309
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1580992..1591837

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B5S52_RS07195 (B5S52_07195)	gspI	1581384..1581749 (+)	366	WP_039284733.1	type II secretion system minor pseudopilin GspI	-
B5S52_RS07200 (B5S52_07200)	gspJ	1581749..1582399 (+)	651	WP_072036238.1	type II secretion system minor pseudopilin GspJ	-
B5S52_RS07205 (B5S52_07205)	gspK	1582396..1583382 (+)	987	WP_039520419.1	type II secretion system minor pseudopilin GspK	-
B5S52_RS07210 (B5S52_07210)	gspL	1583379..1584656 (+)	1278	WP_040038051.1	type II secretion system protein GspL	-
B5S52_RS07215 (B5S52_07215)	gspM	1584653..1585147 (+)	495	WP_040043679.1	type II secretion system protein GspM	-
B5S52_RS07220 (B5S52_07220)	-	1585137..1585883 (+)	747	WP_080163500.1	type II secretion system protein N	-
B5S52_RS07225 (B5S52_07225)	pilD	1585992..1586837 (+)	846	WP_040038049.1	prepilin peptidase	Machinery gene
B5S52_RS22345	ypdK	1586998..1587072 (-)	75	WP_099606229.1	membrane protein YpdK	-
B5S52_RS07230 (B5S52_07230)	-	1587430..1588455 (-)	1026	WP_039503002.1	LLM class flavin-dependent oxidoreductase	-
B5S52_RS07235 (B5S52_07235)	-	1588765..1589829 (+)	1065	WP_080163502.1	ABC transporter substrate-binding protein	-
B5S52_RS07240 (B5S52_07240)	-	1589826..1590863 (+)	1038	WP_080163504.1	FecCD family ABC transporter permease	-
B5S52_RS07245 (B5S52_07245)	-	1590865..1591635 (+)	771	WP_039284758.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31405.37 Da
• Isoelectric Point: 5.6762

>NTDB_id=221562 B5S52_RS07225 WP_040038049.1 1585992..1586837(+) (pilD) [Pectobacterium brasiliense strain SX309]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLEHRWRQDVELETGMVDSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLIDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=221562 B5S52_RS07225 WP_040038049.1 1585992..1586837(+) (pilD) [Pectobacterium brasiliense strain SX309]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCATCGCTGGCGGCAGGATGTCGAGCTTGAAACGG
GCATGGTCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCGTGCCCACACTGCCAGCAGGCTATT
GCAGTGAAGGATAACATCCCGCTATTCAGTTGGCTGTGGTTGCGTGGCCGCTCCCGCTGCTGTCATCAATCAGTATCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTACCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCATTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACCTTTGTCCCATTGATTGATGCCGTAGTCGGCGC
GATGGCTGGTTATTTATCTCTGTGGCTACTTTATTGGGTATTCAAATACGCAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCGTTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTTGTTACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGAGGCGTGTTTGGCGTGATAATGAACGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.815	96.441	0.48
pilD	Neisseria gonorrhoeae MS11	46.743	92.883	0.434
pilD	Vibrio campbellii strain DS40M4	44.981	95.73	0.431
pilD	Acinetobacter baumannii D1279779	41.392	97.153	0.402
pilD	Acinetobacter nosocomialis M2	41.026	97.153	0.399