Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   B5M06_RS06645 Genome accession   NZ_CP020121
Coordinates   1426186..1427775 (+) Length   529 a.a.
NCBI ID   WP_054065353.1    Uniprot ID   A0A1V0BDK4
Organism   Comamonas kerstersii strain 8943     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1421186..1432775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5M06_RS06620 (B5M06_06620) argS 1421531..1423237 (+) 1707 WP_054065349.1 arginine--tRNA ligase -
  B5M06_RS06625 (B5M06_06625) - 1423280..1423918 (+) 639 WP_054065350.1 SPOR domain-containing protein -
  B5M06_RS06630 (B5M06_06630) - 1423974..1424618 (+) 645 WP_054065351.1 thiol:disulfide interchange protein DsbA/DsbL -
  B5M06_RS06635 (B5M06_06635) lptA 1424720..1425355 (+) 636 WP_054065362.1 lipopolysaccharide transport periplasmic protein LptA -
  B5M06_RS06640 (B5M06_06640) lptB 1425355..1426125 (+) 771 WP_054065352.1 LPS export ABC transporter ATP-binding protein -
  B5M06_RS06645 (B5M06_06645) rpoN/rpoN1 1426186..1427775 (+) 1590 WP_054065353.1 RNA polymerase factor sigma-54 Machinery gene
  B5M06_RS06650 (B5M06_06650) - 1427950..1428894 (+) 945 WP_054065354.1 sugar ABC transporter substrate-binding protein -
  B5M06_RS06655 (B5M06_06655) - 1428904..1430475 (+) 1572 WP_054065355.1 sugar ABC transporter ATP-binding protein -
  B5M06_RS06660 (B5M06_06660) - 1430493..1431476 (+) 984 WP_054065356.1 ABC transporter permease -
  B5M06_RS06665 (B5M06_06665) rbsK 1431489..1432400 (+) 912 WP_054065357.1 ribokinase -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58831.61 Da        Isoelectric Point: 4.9801

>NTDB_id=221361 B5M06_RS06645 WP_054065353.1 1426186..1427775(+) (rpoN/rpoN1) [Comamonas kerstersii strain 8943]
MKPSLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLEWSAEEVEREAFGVEHANSAPEADVHASDETYF
SVADSAVSTGAEGQKDSEIPVAEAADLPQDWEGDGSVDMGALDDAEWGGEAPARGKRNEDEDAPSALELARCHESLTDHL
HQQALSLRLSEVQRCALRFLIENLNDDGYLEDSLQELAIGLAGSEDEEQLEELMHHFTVALRLLQSLEPVGVGARDLAEC
LRLQLRYLQEQGGDDPALLEAALQLCAQPLDMLARRDVRKLMQACGLPESRVRAALALIARLEPKPGRRFVDVERHVIVP
DVIVQKTNRKGGDGLMQFSVQLNHDVMPRLRVHEVYAGALRSHKGSEGHAMLQQRLQEARWFIKNVQQRFDTILRVSKAI
VERQKNFFIHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPHGTYELKFFFGSGLGTETGGNASSTAVRALIRQF
VSAENPKKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=221361 B5M06_RS06645 WP_054065353.1 1426186..1427775(+) (rpoN/rpoN1) [Comamonas kerstersii strain 8943]
TTGAAGCCAAGTTTGTCACTGCGGGTCTCACAACATCTGGCGTTGACGCCGCAACTTCAACAATCTATTCGACTGCTGCA
GCTGTCGACCCTGGAGCTTTCTCAGGAAGTCGAGCAGATGCTCGACGAAAACCCATTTCTTGAATGGTCTGCCGAAGAGG
TCGAACGCGAGGCCTTTGGCGTGGAGCATGCAAATTCCGCGCCAGAGGCGGATGTGCATGCCAGCGACGAAACTTATTTT
TCTGTAGCTGACAGCGCCGTATCCACGGGCGCTGAAGGGCAAAAAGATTCAGAAATTCCCGTGGCAGAAGCTGCCGATCT
GCCCCAGGACTGGGAGGGCGATGGCAGTGTAGACATGGGGGCGTTGGATGACGCAGAGTGGGGTGGTGAAGCACCGGCAC
GCGGCAAGCGCAATGAGGACGAGGATGCCCCTTCTGCACTGGAACTGGCCCGCTGCCACGAATCTCTGACGGACCATCTG
CACCAGCAGGCACTGTCGCTGCGCCTGTCGGAAGTGCAGCGCTGCGCCCTGCGCTTTCTGATTGAAAACCTCAACGACGA
CGGCTATCTGGAAGACTCGCTGCAAGAGCTGGCTATTGGCCTGGCAGGCAGTGAAGATGAAGAACAGCTTGAGGAGCTGA
TGCACCACTTCACGGTGGCGCTGCGTCTGCTGCAGTCGCTGGAACCCGTGGGTGTGGGGGCGCGTGATCTGGCCGAATGC
CTGCGCCTGCAGCTGCGCTATCTGCAGGAGCAAGGCGGTGACGACCCTGCGCTGCTGGAAGCCGCCTTGCAGCTGTGTGC
CCAGCCGCTGGACATGCTGGCGCGCCGTGATGTGCGCAAGCTCATGCAGGCCTGTGGTCTGCCTGAAAGCCGCGTGCGCG
CAGCGCTGGCGCTGATTGCACGGCTGGAGCCCAAGCCCGGGCGGCGCTTTGTGGATGTGGAGCGGCATGTGATCGTGCCC
GATGTGATCGTGCAGAAAACGAACCGCAAGGGGGGCGATGGCCTGATGCAGTTCAGCGTGCAGCTCAACCACGATGTGAT
GCCGCGTCTGCGTGTGCACGAGGTCTATGCCGGTGCGCTGCGCAGCCACAAGGGCAGCGAGGGCCACGCCATGCTGCAGC
AGCGCCTGCAGGAGGCGCGCTGGTTCATCAAGAACGTGCAGCAGCGCTTTGACACCATCTTGCGTGTGTCCAAAGCCATT
GTGGAGCGGCAGAAAAACTTCTTTATCCACGGTGAGCTGGCCATGCGTCCGCTGGTGCTGCGCGATATTGCGGACGAGCT
GGGGCTGCACGAATCCACCATCAGCCGTGTGACGACGGCCAAGTACATGGCCACGCCCCACGGCACCTATGAGCTGAAGT
TCTTCTTCGGTTCCGGCCTGGGTACAGAGACGGGCGGCAATGCGTCGAGTACGGCAGTGCGTGCGCTGATCCGCCAGTTT
GTGAGTGCAGAGAACCCCAAAAAACCGCTGTCGGACAGCCAGATTGCCGAGATGCTCAAGGAGCAGGGCATCGAATGTGC
CCGCCGCACGGTGGCGAAATACCGGGAGGCGCTGAAAATTGCGCCAGCAAATTTGAGAAAAGCGTTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-356)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0BDK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.166

100

0.524

Multiple sequence alignment