Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   B5D75_RS05495 Genome accession   NZ_CP020045
Coordinates   1073146..1074096 (+) Length   316 a.a.
NCBI ID   WP_002853794.1    Uniprot ID   -
Organism   Campylobacter jejuni subsp. jejuni strain ATCC 35925     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1059199..1078908 1073146..1074096 within 0


Gene organization within MGE regions


Location: 1059199..1078908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D75_RS05430 (B5D75_05430) pglI 1059199..1060128 (-) 930 WP_044306099.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  B5D75_RS05435 (B5D75_05435) pglH 1060121..1061200 (-) 1080 WP_079005319.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  B5D75_RS05440 (B5D75_05440) pglK 1061197..1062891 (-) 1695 WP_044306101.1 ABC-type lipopolysaccharide transporter PglK -
  B5D75_RS05445 (B5D75_05445) galE 1062885..1063871 (-) 987 WP_002874249.1 UDP-glucose 4-epimerase GalE -
  B5D75_RS05450 (B5D75_05450) - 1063930..1064724 (-) 795 WP_044306102.1 3'-5' exonuclease -
  B5D75_RS05455 (B5D75_05455) waaC 1064790..1065818 (+) 1029 WP_044306103.1 lipopolysaccharide heptosyltransferase I -
  B5D75_RS05460 (B5D75_05460) htrB 1065808..1066695 (+) 888 WP_044306104.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  B5D75_RS05465 (B5D75_05465) - 1066692..1068242 (+) 1551 WP_079005320.1 glycosyltransferase family 2 protein -
  B5D75_RS05470 (B5D75_05470) - 1068211..1069098 (-) 888 WP_052803913.1 glycosyltransferase family 2 protein -
  B5D75_RS05475 (B5D75_05475) - 1069091..1070299 (-) 1209 WP_044306107.1 glycosyltransferase family 2 protein -
  B5D75_RS08445 - 1070377..1070784 (-) 408 WP_052500184.1 hypothetical protein -
  B5D75_RS08530 - 1071023..1071344 (-) 322 Protein_1054 glycosyltransferase family 2 protein -
  B5D75_RS05485 (B5D75_05485) - 1071337..1072224 (-) 888 WP_052803914.1 glycosyltransferase -
  B5D75_RS05490 (B5D75_05490) waaV 1072278..1073087 (-) 810 WP_044306108.1 glycosyltransferase family 2 protein -
  B5D75_RS05495 (B5D75_05495) waaF 1073146..1074096 (+) 951 WP_002853794.1 glycosyltransferase family 9 protein Regulator
  B5D75_RS05500 (B5D75_05500) - 1074082..1074846 (-) 765 WP_044306109.1 glycosyltransferase family 25 protein -
  B5D75_RS05505 (B5D75_05505) gmhA 1074846..1075406 (-) 561 WP_023890815.1 D-sedoheptulose 7-phosphate isomerase -
  B5D75_RS05510 (B5D75_05510) rfaE1 1075403..1076788 (-) 1386 WP_044306110.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  B5D75_RS05515 (B5D75_05515) rfaD 1076781..1077734 (-) 954 WP_044306111.1 ADP-glyceromanno-heptose 6-epimerase -
  B5D75_RS05520 (B5D75_05520) gmhB 1077735..1078295 (-) 561 WP_002853963.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  B5D75_RS05525 (B5D75_05525) - 1078377..1078679 (+) 303 WP_002852762.1 c-type cytochrome -
  B5D75_RS05530 (B5D75_05530) ccoS 1078702..1078908 (-) 207 WP_002877126.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36225.73 Da        Isoelectric Point: 10.2421

>NTDB_id=220648 B5D75_RS05495 WP_002853794.1 1073146..1074096(+) (waaF) [Campylobacter jejuni subsp. jejuni strain ATCC 35925]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDVSYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENATLVHLDLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLTNI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=220648 B5D75_RS05495 WP_002853794.1 1073146..1074096(+) (waaF) [Campylobacter jejuni subsp. jejuni strain ATCC 35925]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCGCCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCTCAGTTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGCAAAATCGATTTTAGCTTTGCT
TTTAGGTCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAGCAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGTGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATTGCTTTTTGTGATCTTTTCATCACAAACGACAGT
GGACCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAACATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

93.61

99.051

0.927

Multiple sequence alignment