Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   Vp2S01_RS01795 Genome accession   NZ_CP020034
Coordinates   342632..343648 (+) Length   338 a.a.
NCBI ID   WP_031855783.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20130629002S01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337632..348648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Vp2S01_RS01780 (Vp2S01_0307) - 338056..338784 (-) 729 WP_031855782.1 redoxin family protein -
  Vp2S01_RS01785 (Vp2S01_0308) oxyR 338955..339863 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  Vp2S01_RS01790 (Vp2S01_0309) - 339919..342489 (-) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  Vp2S01_RS01795 (Vp2S01_0310) pilM 342632..343648 (+) 1017 WP_031855783.1 type IV pilus assembly protein PilM Machinery gene
  Vp2S01_RS01800 (Vp2S01_0311) pilN 343632..344210 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  Vp2S01_RS01805 (Vp2S01_0312) pilO 344203..344796 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  Vp2S01_RS01810 (Vp2S01_0313) pilP 344786..345301 (+) 516 WP_015297395.1 pilus assembly protein PilP Machinery gene
  Vp2S01_RS01815 (Vp2S01_0314) pilQ 345333..347081 (+) 1749 WP_031855784.1 type IV pilus secretin PilQ family protein Machinery gene
  Vp2S01_RS01820 (Vp2S01_0315) aroK 347270..347788 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37789.40 Da        Isoelectric Point: 7.2506

>NTDB_id=220562 Vp2S01_RS01795 WP_031855783.1 342632..343648(+) (pilM) [Vibrio parahaemolyticus strain 20130629002S01]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIKQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEAMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=220562 Vp2S01_RS01795 WP_031855783.1 342632..343648(+) (pilM) [Vibrio parahaemolyticus strain 20130629002S01]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCAAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAATTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGCGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACTATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment