Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B4V02_RS09240 Genome accession   NZ_CP020028
Coordinates   2061131..2061568 (+) Length   145 a.a.
NCBI ID   WP_094154569.1    Uniprot ID   A0A222WM42
Organism   Paenibacillus kribbensis strain AM49     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2056131..2066568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4V02_RS09215 (B4V02_09230) - 2056938..2057606 (+) 669 WP_094154566.1 uracil-DNA glycosylase -
  B4V02_RS09220 (B4V02_09235) - 2057623..2058045 (+) 423 WP_007431083.1 hypothetical protein -
  B4V02_RS09225 (B4V02_09240) - 2058165..2060042 (+) 1878 WP_094154567.1 Ig-like domain-containing protein -
  B4V02_RS09230 (B4V02_09245) - 2060112..2060414 (-) 303 WP_007431081.1 hypothetical protein -
  B4V02_RS09235 (B4V02_09250) - 2060797..2060991 (-) 195 WP_094154568.1 hypothetical protein -
  B4V02_RS09240 (B4V02_09255) nucA/comI 2061131..2061568 (+) 438 WP_094154569.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  B4V02_RS09245 (B4V02_09260) - 2061590..2061796 (-) 207 WP_094154570.1 hypothetical protein -
  B4V02_RS09250 (B4V02_09265) - 2061911..2063731 (+) 1821 WP_094154571.1 sensor histidine kinase -
  B4V02_RS09255 (B4V02_09270) - 2063706..2065331 (+) 1626 WP_094154572.1 response regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16137.42 Da        Isoelectric Point: 9.4113

>NTDB_id=220470 B4V02_RS09240 WP_094154569.1 2061131..2061568(+) (nucA/comI) [Paenibacillus kribbensis strain AM49]
MKKKLLSFITVVLLAAGMYWFKGVDLLTKTPQENPPSSVQVTLHFPSDRYPETAQHIKEAIQTGKSSVCTIDREGAEQNR
KHSLAGVPARKGFDRDEWPMAMCSEGGKGANVKYIAPKDNRGAGSWVSHQLDEYEDGTRVKFVVK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=220470 B4V02_RS09240 WP_094154569.1 2061131..2061568(+) (nucA/comI) [Paenibacillus kribbensis strain AM49]
TTGAAGAAAAAACTGTTGAGTTTTATAACCGTAGTGCTATTGGCCGCGGGTATGTATTGGTTTAAAGGAGTCGATCTCCT
TACGAAAACACCACAAGAGAACCCGCCCTCATCGGTACAGGTTACACTGCATTTTCCGTCGGATCGGTATCCAGAAACGG
CGCAGCATATCAAGGAAGCGATTCAGACGGGAAAATCATCGGTATGCACAATTGATCGTGAAGGAGCCGAGCAAAATCGC
AAGCATTCGCTTGCAGGAGTTCCCGCTCGTAAAGGCTTCGATCGTGACGAATGGCCGATGGCGATGTGCTCAGAAGGTGG
TAAAGGTGCAAATGTCAAATACATAGCTCCTAAGGATAATCGTGGGGCAGGATCATGGGTAAGTCACCAATTGGATGAGT
ATGAGGATGGAACTCGTGTAAAATTTGTCGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222WM42

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.308

71.724

0.483

Multiple sequence alignment