Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CJE_RS06395 Genome accession   NC_003912
Coordinates   1200904..1201854 (+) Length   316 a.a.
NCBI ID   WP_011049891.1    Uniprot ID   -
Organism   Campylobacter jejuni RM1221     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1186952..1214840 1200904..1201854 within 0


Gene organization within MGE regions


Location: 1186952..1214840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJE_RS06330 (CJE1270) pglI 1186952..1187881 (-) 930 WP_002867212.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  CJE_RS06335 (CJE1271) pglH 1187874..1188953 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  CJE_RS06340 (CJE1272) pglK 1188950..1190644 (-) 1695 WP_002867213.1 ABC-type lipopolysaccharide transporter PglK -
  CJE_RS06345 (CJE1273) galE 1190638..1191624 (-) 987 WP_002867214.1 UDP-glucose 4-epimerase GalE -
  CJE_RS06350 (CJE1274) - 1191683..1192477 (-) 795 WP_002860221.1 3'-5' exonuclease -
  CJE_RS06355 (CJE1275) waaC 1192544..1193572 (+) 1029 WP_011049885.1 lipopolysaccharide heptosyltransferase I -
  CJE_RS06360 (CJE1276) htrB 1193562..1194449 (+) 888 WP_002867215.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  CJE_RS06365 (CJE1277) - 1194446..1195996 (+) 1551 WP_002867216.1 glycosyltransferase family 2 protein -
  CJE_RS06370 (CJE1278) - 1195965..1196852 (-) 888 WP_011049886.1 glycosyltransferase family 2 protein -
  CJE_RS06375 (CJE1279) - 1196845..1198053 (-) 1209 WP_011049887.1 glycosyltransferase family 2 protein -
  CJE_RS06380 (CJE1280) - 1198131..1199102 (-) 972 WP_011049888.1 glycosyltransferase family 2 protein -
  CJE_RS06385 (CJE1281) - 1199095..1199982 (-) 888 WP_011049889.1 glycosyltransferase -
  CJE_RS06390 (CJE1282) waaV 1200036..1200845 (-) 810 WP_011049890.1 glycosyltransferase family 2 protein -
  CJE_RS06395 (CJE1283) waaF 1200904..1201854 (+) 951 WP_011049891.1 lipopolysaccharide heptosyltransferase II Regulator
  CJE_RS06400 (CJE1284) - 1201840..1202604 (-) 765 WP_002884483.1 glycosyltransferase family 25 protein -
  CJE_RS06405 (CJE1285) gmhA 1202604..1203164 (-) 561 WP_002869619.1 D-sedoheptulose 7-phosphate isomerase -
  CJE_RS06410 (CJE1286) rfaE1 1203161..1204546 (-) 1386 WP_002867399.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  CJE_RS06415 (CJE1287) rfaD 1204539..1205492 (-) 954 WP_002867398.1 ADP-glyceromanno-heptose 6-epimerase -
  CJE_RS06420 (CJE1288) gmhB 1205493..1206054 (-) 562 Protein_1248 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  CJE_RS06425 (CJE1289) - 1206135..1206437 (+) 303 WP_002852762.1 c-type cytochrome -
  CJE_RS06430 (CJE1290) ccoS 1206460..1206666 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  CJE_RS06435 (CJE1291) - 1206663..1209020 (-) 2358 WP_079254352.1 heavy metal translocating P-type ATPase -
  CJE_RS06440 (CJE1292) rho 1209129..1210427 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  CJE_RS06445 (CJE1293) dnaX 1210431..1211960 (+) 1530 WP_002867395.1 DNA polymerase III subunit gamma/tau -
  CJE_RS10260 (CJE1294) - 1211957..1212475 (-) 519 Protein_1254 DUF2165 family protein -
  CJE_RS06465 (CJE1295) copA 1212547..1214646 (-) 2100 WP_002867392.1 copper-translocating P-type ATPase CopA -
  CJE_RS06470 (CJE1296) - 1214646..1214840 (-) 195 WP_002852760.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36213.76 Da        Isoelectric Point: 10.2878

>NTDB_id=22046 CJE_RS06395 WP_011049891.1 1200904..1201854(+) (waaF) [Campylobacter jejuni RM1221]
MKIFIHLPTWLGDAVMASPALYVIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLHIKAHLKDLKLPFKLKFQSPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENATLVHLNLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLTNI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=22046 CJE_RS06395 WP_011049891.1 1200904..1201854(+) (waaF) [Campylobacter jejuni RM1221]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGTTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTCACATAAAAGCTCACCTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAGCCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAATCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGCCCTATGCACATAAGTGCAGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACAAAATTTACTCAAACCTCACC
TTGGCAAAATGAAAACGCAACATTAGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.652

99.051

0.918

Multiple sequence alignment