Detailed information    

insolico Bioinformatically predicted

Overview


Name   nin/comJ   Type   Regulator
Locus tag   BS21228_RS14220 Genome accession   NZ_CP020023
Coordinates   2647371..2647769 (-) Length   132 a.a.
NCBI ID   WP_014478800.1    Uniprot ID   A0A0D1L5A4
Organism   Bacillus subtilis strain ATCC 21228     
Function   represses the expression of nucA (predicted from homology)   
Competence regulation

Genomic Context


Location: 2642371..2652769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS21228_RS22845 - 2643121..2643318 (+) 198 WP_121509395.1 hypothetical protein -
  BS21228_RS14190 (BS21228_14150) yckC 2643338..2643793 (+) 456 WP_014478793.1 RDD family protein -
  BS21228_RS14195 (BS21228_14155) yckD 2643875..2644207 (+) 333 WP_014478794.1 YckD family protein -
  BS21228_RS14200 (BS21228_14160) - 2644335..2644757 (+) 423 WP_014478795.1 MarR family transcriptional regulator -
  BS21228_RS14205 (BS21228_14165) - 2644745..2645065 (+) 321 WP_014478796.1 DUF3147 family protein -
  BS21228_RS14210 (BS21228_14170) - 2645084..2645828 (+) 745 Protein_2765 AAA family ATPase -
  BS21228_RS14215 (BS21228_14175) bglC 2645901..2647334 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  BS21228_RS14220 (BS21228_14180) nin/comJ 2647371..2647769 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  BS21228_RS14225 (BS21228_14185) nucA/comI 2647796..2648245 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  BS21228_RS14230 (BS21228_14190) tlpC 2648413..2650134 (-) 1722 Protein_2769 methyl-accepting chemotaxis protein TlpC -
  BS21228_RS14235 (BS21228_14195) hxlB 2650245..2650802 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  BS21228_RS14240 (BS21228_14200) hxlA 2650808..2651440 (-) 633 WP_014478804.1 3-hexulose-6-phosphate synthase -
  BS21228_RS14245 (BS21228_14205) hxlR 2651669..2652031 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence


Protein


Download         Length: 132 a.a.        Molecular weight: 15006.98 Da        Isoelectric Point: 4.8991

>NTDB_id=220348 BS21228_RS14220 WP_014478800.1 2647371..2647769(-) (nin/comJ) [Bacillus subtilis strain ATCC 21228]
MIKSWKPQELSISYHQFTVFQKDSTPPIMDWTDEAIEKGYTAADGAISFEAQRNTKAFILLRLNSSETVNSYEKKVTVPF
HVTENGIHIESIMSKRLSFDLPKGDYQLTCWTVPAEMSDLHADTYIIDAVSV

Nucleotide


Download         Length: 399 bp        

>NTDB_id=220348 BS21228_RS14220 WP_014478800.1 2647371..2647769(-) (nin/comJ) [Bacillus subtilis strain ATCC 21228]
TTGATCAAATCATGGAAGCCGCAAGAACTTTCGATTTCATATCATCAATTTACTGTATTTCAAAAGGATTCTACACCTCC
TATAATGGATTGGACTGACGAAGCGATTGAAAAAGGATATACTGCGGCAGACGGAGCCATTTCATTTGAGGCACAGCGAA
ATACAAAGGCCTTTATTCTTTTGCGCCTGAACAGTTCAGAAACAGTAAACTCCTACGAAAAAAAAGTGACTGTTCCTTTT
CATGTCACAGAAAACGGAATTCATATTGAAAGCATTATGTCTAAGAGACTGTCCTTTGATCTGCCTAAAGGAGACTATCA
ATTGACATGCTGGACTGTGCCGGCTGAAATGTCAGATTTGCACGCCGACACTTATATTATTGATGCTGTTTCTGTGTAA

Domains


Predicted by InterproScan.

(9-129)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0D1L5A4

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nin/comJ Bacillus subtilis subsp. subtilis str. 168

97.727

100

0.977


Multiple sequence alignment