Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BS21228_RS03345 Genome accession   NZ_CP020023
Coordinates   582725..583492 (-) Length   255 a.a.
NCBI ID   WP_014480292.1    Uniprot ID   -
Organism   Bacillus subtilis strain ATCC 21228     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 577725..588492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS21228_RS03325 (BS21228_03290) ppsR 577900..578712 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  BS21228_RS03330 (BS21228_03295) ccpN 578738..579376 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  BS21228_RS03335 (BS21228_03300) glyS 579509..581548 (-) 2040 WP_014480291.1 glycine--tRNA ligase subunit beta -
  BS21228_RS03340 (BS21228_03305) glyQ 581541..582428 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  BS21228_RS03345 (BS21228_03310) recO 582725..583492 (-) 768 WP_014480292.1 DNA repair protein RecO Machinery gene
  BS21228_RS03350 (BS21228_03315) yqzL 583529..583672 (-) 144 WP_003230053.1 YqzL family protein -
  BS21228_RS03355 (BS21228_03320) era 583820..584725 (-) 906 WP_003230051.1 GTPase Era -
  BS21228_RS03360 (BS21228_03325) - 584706..585116 (-) 411 WP_014480293.1 cytidine deaminase -
  BS21228_RS03365 (BS21228_03330) unpK 585235..585606 (-) 372 WP_014480294.1 diacylglycerol kinase family protein -
  BS21228_RS03370 (BS21228_03335) ybeY 585587..586060 (-) 474 WP_014480295.1 rRNA maturation RNase YbeY -
  BS21228_RS03375 (BS21228_03340) pgpH 586061..588196 (-) 2136 WP_014480296.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=220311 BS21228_RS03345 WP_014480292.1 582725..583492(-) (recO) [Bacillus subtilis strain ATCC 21228]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPLKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=220311 BS21228_RS03345 WP_014480292.1 582725..583492(-) (recO) [Bacillus subtilis strain ATCC 21228]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAGAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGCTCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment