Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B2J85_RS11005 Genome accession   NZ_CP020017
Coordinates   2292777..2294147 (-) Length   456 a.a.
NCBI ID   WP_014123419.1    Uniprot ID   -
Organism   Tetragenococcus halophilus strain KUD23     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2287777..2299147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2J85_RS10985 - 2287980..2288369 (-) 390 WP_014123423.1 Mini-ribonuclease 3 -
  B2J85_RS10990 cysS 2288366..2289775 (-) 1410 WP_014123422.1 cysteine--tRNA ligase -
  B2J85_RS10995 gltX 2290032..2291489 (-) 1458 WP_094244078.1 glutamate--tRNA ligase -
  B2J85_RS11000 - 2291616..2292752 (-) 1137 WP_041591731.1 PIN/TRAM domain-containing protein -
  B2J85_RS11005 radA 2292777..2294147 (-) 1371 WP_014123419.1 DNA repair protein RadA Machinery gene
  B2J85_RS11010 - 2294171..2294671 (-) 501 WP_094244079.1 dUTP diphosphatase -
  B2J85_RS11015 - 2294867..2295757 (+) 891 WP_198362431.1 aldo/keto reductase -
  B2J85_RS11020 proB 2296008..2296814 (+) 807 WP_014123416.1 glutamate 5-kinase -
  B2J85_RS11025 - 2296811..2298058 (+) 1248 WP_014123415.1 glutamate-5-semialdehyde dehydrogenase -
  B2J85_RS11030 - 2298239..2298436 (+) 198 WP_014123414.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50003.59 Da        Isoelectric Point: 7.0761

>NTDB_id=220147 B2J85_RS11005 WP_014123419.1 2292777..2294147(-) (radA) [Tetragenococcus halophilus strain KUD23]
MAKKVKTQFECQNCGYISPRYLGKCPNCGSWNSMVEEKLQDTTDRRNRTTLTGSKMQPTLLKDVVPKKEPRVKTSLDELN
RVLGGGVVPGSMILLGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAQRLGDAENDFYLFAETGMQDIRE
TIEKLQPDYVIIDSIQTMTQPDISSVAGSVSQVRETTAELLKIAKSNEIAVFVVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKYQSFRILRAVKNRFGSTNEIGIFEMYEQGLKEVSNPSQVFLEERLEGTSGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTATGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAISIASSYKEKGTKPTECFIGEIGLTG
EIRRVSAIEQRVKEVQKLGFEKVYIPKNNLSGWKAPADIEVVGIATLDEALKRIFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=220147 B2J85_RS11005 WP_014123419.1 2292777..2294147(-) (radA) [Tetragenococcus halophilus strain KUD23]
ATGGCAAAAAAGGTAAAAACACAATTTGAATGTCAAAATTGCGGTTATATTTCACCTAGGTATCTAGGCAAATGTCCTAA
TTGTGGTAGTTGGAATTCGATGGTAGAAGAAAAACTACAAGACACTACCGATCGCAGGAATCGAACAACACTGACAGGTA
GCAAGATGCAGCCGACACTTTTAAAAGATGTTGTTCCTAAAAAAGAGCCACGAGTAAAGACATCTCTAGATGAATTAAAC
CGTGTCTTAGGTGGAGGTGTGGTTCCAGGATCGATGATTCTATTAGGCGGGGATCCTGGTATTGGTAAGTCCACCTTGCT
TTTACAAGTATCGCAACAGTTAGCAGCAACCGGTGGAACAGTACTTTATGTCTCCGGAGAAGAAAGTGCAGAACAAATCA
AATTACGAGCTCAGCGGTTAGGCGATGCCGAAAATGATTTTTATTTATTTGCTGAAACGGGTATGCAAGATATTCGAGAA
ACAATTGAAAAGCTGCAACCTGATTATGTGATCATTGACTCCATCCAAACGATGACACAACCAGATATATCAAGCGTGGC
CGGAAGTGTTAGTCAAGTTCGAGAAACTACGGCCGAGTTATTAAAAATTGCTAAATCAAACGAAATTGCCGTCTTTGTGG
TTGGCCATGTGACTAAAGAAGGTTCTATCGCTGGGCCTCGTATGCTAGAGCATATGGTGGATACGGTATTGTATTTTGAA
GGGGATAAGTACCAAAGCTTTCGTATTTTAAGGGCAGTAAAAAACCGTTTTGGTTCGACCAATGAGATCGGCATTTTTGA
AATGTATGAACAAGGCCTCAAAGAAGTAAGTAATCCTTCACAGGTTTTTTTAGAAGAACGATTAGAAGGAACTAGCGGAT
CTGCCATTGTGGTTGCGATGGAAGGAACGCGTCCTATTCTAGTGGAAATACAAGCTTTAGTTACACCAACGATGTTTGGA
AATGCTAAACGAACGGCTACTGGCTTGGATTTTAATCGAGTTTCTTTGATTATGGCTGTATTAGAAAAACGGGCAGGTCT
ACTTTTACAAAACCAGGATGCTTATTTAAAAGCAGCTGGTGGTGTAAAATTAAATGAACCAGCGATCGATCTAGCTATAG
CTATTAGTATTGCTTCAAGTTATAAAGAAAAAGGCACAAAACCTACTGAATGTTTCATTGGAGAAATTGGTCTTACAGGG
GAAATTCGTCGCGTGAGTGCTATTGAGCAGCGCGTAAAAGAGGTTCAAAAATTAGGTTTTGAGAAAGTATATATACCAAA
AAATAATTTAAGCGGCTGGAAAGCGCCAGCTGACATCGAGGTTGTTGGTATAGCAACACTTGATGAAGCATTAAAACGTA
TATTTAGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(71-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

70.33

99.781

0.702
  radA Streptococcus mitis NCTC 12261

70.33

99.781

0.702
  radA Streptococcus pneumoniae Rx1

70.33

99.781

0.702
  radA Streptococcus pneumoniae D39

70.33

99.781

0.702
  radA Streptococcus pneumoniae R6

70.33

99.781

0.702
  radA Streptococcus pneumoniae TIGR4

70.33

99.781

0.702
  radA Bacillus subtilis subsp. subtilis str. 168

63.858

98.904

0.632

Multiple sequence alignment