Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OTEC02_RS16250 Genome accession   NZ_CP020015
Coordinates   3422442..3423242 (-) Length   266 a.a.
NCBI ID   WP_201783225.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain OTEC-02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3417442..3428242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTEC02_RS16230 (OTEC02_16230) - 3418504..3419202 (-) 699 WP_016144303.1 YfgM family protein -
  OTEC02_RS16235 (OTEC02_16235) hisS 3419231..3420523 (-) 1293 WP_062849551.1 histidine--tRNA ligase -
  OTEC02_RS16240 (OTEC02_16240) ispG 3420520..3421635 (-) 1116 WP_081175305.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  OTEC02_RS16245 (OTEC02_16245) - 3421657..3422451 (-) 795 WP_081175307.1 helix-turn-helix domain-containing protein -
  OTEC02_RS16250 (OTEC02_16250) pilF 3422442..3423242 (-) 801 WP_201783225.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OTEC02_RS16255 (OTEC02_16255) rlmN 3423257..3424492 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  OTEC02_RS16260 (OTEC02_16260) ndk 3424616..3425047 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  OTEC02_RS16265 (OTEC02_16265) iscX 3425161..3425361 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  OTEC02_RS16270 (OTEC02_16270) - 3425461..3426012 (-) 552 WP_016144298.1 phosphatase PAP2 family protein -
  OTEC02_RS16275 (OTEC02_16275) - 3426037..3427323 (-) 1287 WP_080648637.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30046.30 Da        Isoelectric Point: 9.2541

>NTDB_id=220101 OTEC02_RS16250 WP_201783225.1 3422442..3423242(-) (pilF) [Acinetobacter lactucae strain OTEC-02]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKTNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMGRYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
VNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQALVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=220101 OTEC02_RS16250 WP_201783225.1 3422442..3423242(-) (pilF) [Acinetobacter lactucae strain OTEC-02]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCGGAAAAAGCAGTAAAAGTGCGTACACAACTTGCTGCTGAACATATCCGTTCTGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCAACAGCAAACATGATGATGGGGATTTTG
CTACAGCAAGAAGGTAGCAAAACCAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGGGCGTTATAATGATGCAATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GTGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGTGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGCGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.609

100

0.936
  pilF Acinetobacter baylyi ADP1

75.385

97.744

0.737

Multiple sequence alignment