Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BUM88_RS02465 Genome accession   NZ_CP020000
Coordinates   516204..517004 (+) Length   266 a.a.
NCBI ID   WP_003654874.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain CA16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511204..522004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUM88_RS02440 (BUM88_02440) - 512120..513409 (+) 1290 WP_080026276.1 glycosyltransferase family 4 protein -
  BUM88_RS02445 (BUM88_02445) - 513434..513985 (+) 552 WP_003654868.1 phosphatase PAP2 family protein -
  BUM88_RS02450 (BUM88_02450) iscX 514085..514285 (+) 201 WP_003654869.1 Fe-S cluster assembly protein IscX -
  BUM88_RS02455 (BUM88_02455) ndk 514399..514830 (+) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  BUM88_RS02460 (BUM88_02460) rlmN 514954..516189 (+) 1236 WP_005036936.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BUM88_RS02465 (BUM88_02465) pilF 516204..517004 (+) 801 WP_003654874.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BUM88_RS02470 (BUM88_02470) - 516995..517786 (+) 792 WP_080026278.1 helix-turn-helix domain-containing protein -
  BUM88_RS02475 (BUM88_02475) ispG 517808..518923 (+) 1116 WP_004641112.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BUM88_RS02480 (BUM88_02480) hisS 518920..520212 (+) 1293 WP_080026279.1 histidine--tRNA ligase -
  BUM88_RS02485 (BUM88_02485) - 520241..520939 (+) 699 WP_004641116.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30120.30 Da        Isoelectric Point: 9.0417

>NTDB_id=219719 BUM88_RS02465 WP_003654874.1 516204..517004(+) (pilF) [Acinetobacter calcoaceticus strain CA16]
MSTPKLKTVFCMGVAVALLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQSLLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=219719 BUM88_RS02465 WP_003654874.1 516204..517004(+) (pilF) [Acinetobacter calcoaceticus strain CA16]
TTGAGTACTCCAAAGTTAAAAACGGTATTTTGTATGGGAGTTGCAGTTGCGCTTTTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAAAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCAGGTGACTTGG
ATTCGGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
CTACAGCAAGAAGGTAGTAAGCCGAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCAGATAA
TGCTCAAGCACATAATAACTATGGTACTTATTTATACCAAATGGAACGCTATAATGATGCGATCGAGCAATTCCGTATTG
CTGGCACAACATTAGGTTATGACCAACGCTATCAGGCGTTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAGCAATCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAA
TTACGTGCCTTGTTCCCAGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

94.737

100

0.947
  pilF Acinetobacter baylyi ADP1

75.385

97.744

0.737

Multiple sequence alignment