Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LS41612_RS00975 Genome accession   NZ_CP019980
Coordinates   208735..210111 (+) Length   458 a.a.
NCBI ID   WP_024364396.1    Uniprot ID   A0A4U2YVH7
Organism   Lysinibacillus sphaericus strain DSM 28     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 203735..215111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LS41612_RS00955 (LS41612_00955) - 204058..204528 (+) 471 WP_024364400.1 CtsR family transcriptional regulator -
  LS41612_RS00960 (LS41612_00960) - 204542..205084 (+) 543 WP_024364399.1 UvrB/UvrC motif-containing protein -
  LS41612_RS00965 (LS41612_00965) - 205081..206190 (+) 1110 WP_024364398.1 protein arginine kinase -
  LS41612_RS00970 (LS41612_00970) - 206180..208624 (+) 2445 WP_024364397.1 ATP-dependent Clp protease ATP-binding subunit -
  LS41612_RS00975 (LS41612_00975) radA 208735..210111 (+) 1377 WP_024364396.1 DNA repair protein RadA Machinery gene
  LS41612_RS00980 (LS41612_00980) - 210413..211498 (+) 1086 WP_024364395.1 PIN/TRAM domain-containing protein -
  LS41612_RS00985 (LS41612_00985) ispD 211511..212206 (+) 696 WP_024364394.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LS41612_RS00990 (LS41612_00990) ispF 212401..212877 (+) 477 WP_024364393.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  LS41612_RS00995 (LS41612_00995) gltX 213002..214462 (+) 1461 WP_024364392.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50032.83 Da        Isoelectric Point: 7.3824

>NTDB_id=219489 LS41612_RS00975 WP_024364396.1 208735..210111(+) (radA) [Lysinibacillus sphaericus strain DSM 28]
MAKKKTKFVCTGCGYESAKWMGRCPGCGEWNKMVEEVEVVAKGPRGAFQHSATVTQKAVPIIQVEAAEESRVATEMGELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSALLSNKGHRVLYISGEESIRQTKLRAERLGVVSQELYIYSETNLELLNQ
TIDDVQPKFVIVDSIQTVFHPEVTSAPGSVSQVRECTAELMRIAKTKGIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTIVASMEGTRPILVEIQSLVTPTSFN
YPKRMATGVDQNRVQLLMAVLEKRMGLMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVRGTDCFIGEVGLTG
EVRRVTRIEQRVLEAAKLGFKRAFIPASNIGGWDFPQGIEIVGVETIKDALNACFREL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=219489 LS41612_RS00975 WP_024364396.1 208735..210111(+) (radA) [Lysinibacillus sphaericus strain DSM 28]
ATGGCGAAGAAAAAAACTAAATTTGTCTGTACAGGTTGTGGCTATGAATCGGCAAAGTGGATGGGACGTTGTCCAGGATG
TGGTGAATGGAATAAAATGGTTGAGGAAGTGGAGGTTGTGGCGAAAGGCCCTAGAGGAGCATTCCAACACTCTGCAACCG
TCACGCAAAAAGCTGTACCTATTATCCAAGTAGAAGCGGCTGAGGAGTCCCGTGTAGCTACAGAAATGGGTGAACTCAAT
CGTGTCTTGGGCGGAGGAATTGTTCCGGGATCTCTAGTGTTAATCGGGGGTGATCCGGGTATCGGAAAATCGACATTGCT
ATTACAGGTGTCTGCGTTATTGTCCAATAAAGGTCATCGTGTGCTCTATATTTCTGGTGAGGAATCCATTCGACAAACGA
AACTGCGTGCAGAGCGTTTAGGGGTTGTATCTCAAGAGCTGTATATATATTCGGAGACGAATTTAGAATTGTTAAATCAA
ACAATCGACGATGTACAACCAAAGTTTGTTATTGTGGATTCTATCCAAACGGTGTTCCATCCAGAGGTGACAAGTGCGCC
AGGTAGTGTATCGCAAGTACGTGAATGTACAGCGGAGCTAATGCGGATAGCGAAAACAAAAGGCATTGCCATTTTCTTAG
TTGGACATGTGACGAAGGAAGGGCAAATTGCGGGACCGCGTATTTTAGAGCATATGGTAGATACAGTGCTGTATTTCGAA
GGAGAACGACATCACAACCACCGTATTTTACGGAGCCAAAAAAACCGCTTTGGCTCTACAAATGAAATAGCTATCTTTGA
AATGCTTCAGGGGGGCTTAAAGGAAGTTTTGAATCCGTCTGAGCTATTTTTACAGGAACGTTCACAAGGTGCAGCAGGTT
CAACAATCGTGGCATCGATGGAGGGAACAAGGCCAATTTTAGTAGAAATCCAATCACTTGTAACCCCTACAAGCTTTAAT
TATCCGAAACGGATGGCAACAGGGGTGGACCAAAACCGTGTGCAATTGCTTATGGCAGTGCTTGAAAAGCGCATGGGCCT
CATGTTACAGGCACAAGATGCATATATTAAAGTTGCTGGCGGAGTAAAGCTAGATGAACCTGCCATTGACTTAGCTGTTT
TGACGAGTATTGTGTCTAGCTTTAAGGATCAGGCAGTACGGGGAACAGATTGCTTTATTGGAGAAGTAGGGTTAACAGGA
GAGGTTCGTCGTGTCACACGAATTGAACAACGTGTGCTAGAGGCGGCAAAGCTAGGGTTTAAGCGAGCATTTATCCCTGC
TTCTAATATTGGCGGTTGGGATTTCCCACAAGGTATTGAAATAGTTGGTGTGGAAACAATTAAAGATGCATTAAATGCCT
GCTTTAGAGAGCTTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(346-429)

AAA domain (AAA_25)

(65-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U2YVH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.264

99.127

0.697
  radA Streptococcus pneumoniae Rx1

62.281

99.563

0.62
  radA Streptococcus pneumoniae D39

62.281

99.563

0.62
  radA Streptococcus pneumoniae R6

62.281

99.563

0.62
  radA Streptococcus pneumoniae TIGR4

62.281

99.563

0.62
  radA Streptococcus mitis NCTC 12261

62.281

99.563

0.62
  radA Streptococcus mitis SK321

62.281

99.563

0.62

Multiple sequence alignment