Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   CY655_RS12970 Genome accession   NZ_CP025573
Coordinates   2548963..2550390 (+) Length   475 a.a.
NCBI ID   WP_000980589.1    Uniprot ID   P04995
Organism   Escherichia coli strain E-1246     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2543963..2555390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CY655_RS12930 (CY655_13465) yeeW 2544147..2544341 (+) 195 WP_000988600.1 DUF5983 family protein -
  CY655_RS12935 - 2544387..2544467 (+) 81 Protein_2488 hypothetical protein -
  CY655_RS12940 (CY655_13470) - 2544756..2544902 (-) 147 Protein_2489 transposase domain-containing protein -
  CY655_RS12945 (CY655_13475) - 2544956..2545138 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  CY655_RS12950 (CY655_13480) tmaR 2545239..2545568 (-) 330 WP_000450409.1 DUF496 family protein -
  CY655_RS12955 (CY655_13485) yeeA 2545740..2546798 (-) 1059 WP_001200884.1 FUSC family protein -
  CY655_RS12960 (CY655_13490) sbmC 2546996..2547469 (-) 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
  CY655_RS12965 (CY655_13495) dacD 2547588..2548754 (-) 1167 WP_000830160.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  CY655_RS12970 (CY655_13500) sbcB 2548963..2550390 (+) 1428 WP_000980589.1 exodeoxyribonuclease I Machinery gene
  CY655_RS12975 (CY655_13505) tsuB 2550433..2550660 (-) 228 WP_000234896.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  CY655_RS12980 (CY655_13510) tsuA 2550674..2551732 (-) 1059 WP_000492309.1 thiosulfate utilization transporter TsuA/YeeE -
  CY655_RS12985 (CY655_13515) plaP 2551911..2553269 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  CY655_RS12990 (CY655_13520) yoeI 2553259..2553321 (-) 63 WP_010723108.1 membrane protein YoeI -
  CY655_RS12995 (CY655_13525) yeeY 2553536..2554465 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  CY655_RS13000 (CY655_13530) yeeZ 2554511..2555335 (-) 825 WP_077513715.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54500.81 Da        Isoelectric Point: 5.4700

>NTDB_id=219437 CY655_RS12970 WP_000980589.1 2548963..2550390(+) (sbcB) [Escherichia coli strain E-1246]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=219437 CY655_RS12970 WP_000980589.1 2548963..2550390(+) (sbcB) [Escherichia coli strain E-1246]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGAGAACCCGAAGTCTTTTACTGCAAGCCCG
CTGATGACTATTTACCCCAGCCAGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTGCTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGCAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAACGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1FXX
  PDB 2QXF
  PDB 3C94
  PDB 3C95
  PDB 3HL8
  PDB 3HP9
  PDB 4HCB
  PDB 4HCC
  PDB 4JRP
  PDB 4JRQ
  PDB 4JS4
  PDB 4JS5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

98.947

0.585

Multiple sequence alignment