Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C0J56_RS28870 Genome accession   NZ_CP025542
Coordinates   6349468..6351252 (+) Length   594 a.a.
NCBI ID   WP_134926295.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain 2P24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6344468..6356252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0J56_RS28850 (C0J56_28835) argE 6345552..6346709 (-) 1158 WP_134926292.1 acetylornithine deacetylase -
  C0J56_RS28855 (C0J56_28840) - 6346868..6348235 (+) 1368 WP_134926293.1 CYTH domain-containing protein -
  C0J56_RS28860 (C0J56_28845) - 6348388..6348858 (-) 471 WP_038856038.1 Lrp/AsnC family transcriptional regulator -
  C0J56_RS28865 (C0J56_28850) - 6348985..6349323 (+) 339 WP_134926294.1 hypothetical protein -
  C0J56_RS28870 (C0J56_28855) pilB 6349468..6351252 (+) 1785 WP_134926295.1 GspE/PulE family protein Machinery gene
  C0J56_RS28875 (C0J56_28860) - 6351480..6352400 (+) 921 WP_003206668.1 SPFH domain-containing protein -
  C0J56_RS28880 (C0J56_28865) - 6352410..6352859 (+) 450 WP_134926296.1 NfeD family protein -
  C0J56_RS28885 (C0J56_28870) - 6352925..6353245 (+) 321 WP_170846005.1 DUF2388 domain-containing protein -
  C0J56_RS28890 (C0J56_28875) gcvH 6353426..6353809 (-) 384 WP_134926297.1 glycine cleavage system protein GcvH -
  C0J56_RS28895 (C0J56_28880) gcvT 6353861..6354943 (-) 1083 WP_134926298.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 66304.94 Da        Isoelectric Point: 6.8654

>NTDB_id=219401 C0J56_RS28870 WP_134926295.1 6349468..6351252(+) (pilB) [Pseudomonas fluorescens strain 2P24]
MSTKHATQDRWLDLNDLLRQLVAQGFISQDSAETALNARRRHSANAQLHPLEFIANQHLDDLSRPGKRLDLESLTLWLSQ
QAGQPYMRIDPLKINVAAVTPLMSYAFAQRHKILAVAVDRDAVTVASAQPYVNSWEADLTHVLKLPIKRVVANPVDIQRL
SVEFYRLAKSVSGATTVDQQPSNLSNFEQLLNLGASDQEPDANDAHIVNIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHTVYQFPPQVTMAIVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGGEVELRLSTLPTAFGEKMVMRIFDPEVLLKD
FDQLGFSADDLRRWQDMTRQPNGIILVTGPTGSGKTTTLYTTLKKLATPEVNLCTIEDPIEMVEASFNQMQVQHNIDLSF
AAGVRALMRQDPDIIMIGEIRDLETAEMAIQAALTGHLVLSTLHTNDAPSAISRLLELGVPHYLIKATVLGVMAQRLVRT
LCPQCKAPLTLDEDDWQTLTRPWQAPLPSNAQQAVGCLECRDTGYRGRAGVYEIMQLTDSVKALIHPDTDLLAVRRQAFK
EGMRSLRLSGAQKVAAGLTTVEEVLRVTPQSEQK

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=219401 C0J56_RS28870 WP_134926295.1 6349468..6351252(+) (pilB) [Pseudomonas fluorescens strain 2P24]
ATGTCCACCAAACACGCCACTCAGGACCGCTGGCTGGACCTCAACGATCTGCTGCGCCAACTGGTCGCCCAGGGCTTCAT
CAGCCAGGATTCAGCCGAAACCGCGCTTAACGCCCGCCGACGCCACAGCGCCAATGCCCAGCTGCATCCCCTGGAGTTCA
TCGCCAACCAGCACCTGGACGACCTCAGCCGCCCCGGCAAGCGCCTGGACCTGGAGAGCCTGACCCTATGGTTGTCCCAA
CAGGCCGGCCAGCCCTACATGCGTATCGACCCGTTGAAAATCAACGTCGCGGCCGTGACGCCCTTGATGTCCTATGCCTT
TGCCCAGCGCCACAAGATCCTCGCGGTGGCGGTCGATCGTGACGCCGTCACCGTCGCCAGCGCACAACCTTACGTCAACA
GCTGGGAAGCCGACCTGACCCACGTGCTCAAGCTGCCGATCAAGCGGGTGGTCGCCAACCCGGTGGACATCCAGCGCTTG
AGCGTGGAGTTCTATCGCCTGGCCAAATCGGTCAGCGGCGCCACCACGGTCGACCAGCAACCGAGCAACCTGAGCAATTT
CGAACAGTTGCTCAACCTCGGTGCCAGCGACCAGGAGCCGGACGCCAATGACGCCCACATCGTCAATATCGTCGACTGGC
TGTTCCAGTACGCCTTCCAGCAGCGCGCCAGCGATATCCACATCGAACCGCGCCGCGAGCAGGGCACCGTACGGTTTCGC
ATCGACGGGGTGCTGCACACCGTCTATCAGTTCCCGCCGCAGGTCACGATGGCGATCGTCAGCCGCCTCAAGAGCCTGGG
GCGGATGAACGTCGCGGAAAAACGCAAACCCCAGGACGGCCGGGTCAAGACCAAGACCCCGGACGGCGGCGAAGTGGAAT
TGCGCCTGTCGACCTTGCCCACGGCGTTCGGCGAAAAAATGGTCATGCGGATCTTCGACCCGGAAGTATTGCTCAAGGAC
TTCGACCAACTCGGTTTCTCCGCCGACGACCTGCGCCGCTGGCAAGACATGACCCGCCAGCCCAACGGCATCATCCTGGT
GACCGGGCCTACCGGTTCCGGCAAGACCACCACCCTCTACACCACCCTCAAGAAGCTGGCGACGCCGGAAGTGAACCTCT
GCACCATCGAAGACCCGATCGAGATGGTCGAGGCATCCTTCAACCAGATGCAGGTCCAGCACAACATCGACCTGAGCTTC
GCTGCCGGGGTGCGGGCCCTGATGCGACAAGACCCGGACATCATCATGATCGGTGAGATCCGCGACCTGGAGACCGCTGA
AATGGCGATCCAGGCGGCGCTCACCGGGCACCTTGTCCTGTCGACGCTGCACACCAACGACGCGCCGAGCGCCATCAGTC
GCCTGCTGGAGCTGGGTGTGCCCCATTACCTGATCAAGGCCACCGTGCTCGGGGTCATGGCCCAGCGGCTGGTGCGCACC
CTGTGCCCGCAGTGCAAGGCCCCCCTGACCCTGGATGAAGATGACTGGCAGACCCTGACCCGGCCCTGGCAGGCACCGCT
GCCGAGCAATGCCCAACAAGCCGTCGGTTGCCTGGAATGCCGCGACACCGGTTACCGCGGCCGCGCCGGGGTCTACGAGA
TCATGCAACTGACCGACAGCGTCAAGGCCTTGATCCACCCCGACACCGACCTGTTGGCGGTGCGCCGCCAGGCCTTCAAG
GAAGGCATGCGCAGCCTGCGCTTGTCGGGGGCGCAGAAGGTGGCGGCGGGCTTGACCACTGTCGAGGAAGTGCTGCGGGT
GACGCCACAGAGCGAGCAGAAGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(216-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(21-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.51

96.296

0.38
  pilB Acinetobacter baumannii D1279779

36.86

98.653

0.364

Multiple sequence alignment