Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   B2K12_RS05495 Genome accession   NZ_CP019965
Coordinates   1071215..1072165 (+) Length   316 a.a.
NCBI ID   WP_002853794.1    Uniprot ID   -
Organism   Campylobacter jejuni strain NCTC12662     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1057268..1085154 1071215..1072165 within 0


Gene organization within MGE regions


Location: 1057268..1085154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2K12_RS05430 (B2K12_05430) pglI 1057268..1058197 (-) 930 WP_044306099.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  B2K12_RS05435 (B2K12_05435) pglH 1058190..1059269 (-) 1080 WP_079005319.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  B2K12_RS05440 (B2K12_05440) pglK 1059266..1060960 (-) 1695 WP_044306101.1 ABC-type lipopolysaccharide transporter PglK -
  B2K12_RS05445 (B2K12_05445) galE 1060954..1061940 (-) 987 WP_002874249.1 UDP-glucose 4-epimerase GalE -
  B2K12_RS05450 (B2K12_05450) - 1061999..1062793 (-) 795 WP_044306102.1 3'-5' exonuclease -
  B2K12_RS05455 (B2K12_05455) waaC 1062859..1063887 (+) 1029 WP_044306103.1 lipopolysaccharide heptosyltransferase I -
  B2K12_RS05460 (B2K12_05460) htrB 1063877..1064764 (+) 888 WP_044306104.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  B2K12_RS05465 (B2K12_05465) - 1064761..1066311 (+) 1551 WP_079005320.1 glycosyltransferase -
  B2K12_RS05470 (B2K12_05470) - 1066280..1067167 (-) 888 WP_052803913.1 glycosyltransferase family 2 protein -
  B2K12_RS05475 (B2K12_05475) - 1067160..1068368 (-) 1209 WP_044306107.1 glycosyltransferase family 2 protein -
  B2K12_RS08440 - 1068446..1068853 (-) 408 WP_052500184.1 hypothetical protein -
  B2K12_RS08445 - 1068883..1069413 (-) 531 WP_052500185.1 glycosyltransferase family 2 protein -
  B2K12_RS05485 (B2K12_05485) - 1069406..1070293 (-) 888 WP_052803914.1 glycosyltransferase -
  B2K12_RS05490 (B2K12_05490) waaV 1070347..1071156 (-) 810 WP_044306108.1 glycosyltransferase family 2 protein -
  B2K12_RS05495 (B2K12_05495) waaF 1071215..1072165 (+) 951 WP_002853794.1 glycosyltransferase family 9 protein Regulator
  B2K12_RS05500 (B2K12_05500) - 1072151..1072915 (-) 765 WP_044306109.1 glycosyltransferase family 25 protein -
  B2K12_RS05505 (B2K12_05505) gmhA 1072915..1073475 (-) 561 WP_023890815.1 D-sedoheptulose 7-phosphate isomerase -
  B2K12_RS05510 (B2K12_05510) rfaE1 1073472..1074857 (-) 1386 WP_044306110.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  B2K12_RS05515 (B2K12_05515) rfaD 1074850..1075803 (-) 954 WP_044306111.1 ADP-glyceromanno-heptose 6-epimerase -
  B2K12_RS05520 (B2K12_05520) gmhB 1075804..1076364 (-) 561 WP_002853963.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  B2K12_RS05525 (B2K12_05525) - 1076446..1076748 (+) 303 WP_002852762.1 cytochrome c -
  B2K12_RS05530 (B2K12_05530) ccoS 1076771..1076977 (-) 207 WP_002877126.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  B2K12_RS05535 (B2K12_05535) - 1076974..1079331 (-) 2358 WP_079005321.1 heavy metal translocating P-type ATPase -
  B2K12_RS05540 (B2K12_05540) rho 1079440..1080738 (+) 1299 WP_044306113.1 transcription termination factor Rho -
  B2K12_RS05545 (B2K12_05545) dnaX 1080742..1082271 (+) 1530 WP_044306114.1 DNA polymerase III subunit gamma/tau -
  B2K12_RS05550 (B2K12_05550) - 1082268..1082789 (-) 522 WP_002853472.1 DUF2165 family protein -
  B2K12_RS05555 (B2K12_05555) copA 1082861..1084960 (-) 2100 WP_044306115.1 copper-translocating P-type ATPase CopA -
  B2K12_RS05560 (B2K12_05560) - 1084960..1085154 (-) 195 WP_002882808.1 cation transporter -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36225.73 Da        Isoelectric Point: 10.2421

>NTDB_id=219272 B2K12_RS05495 WP_002853794.1 1071215..1072165(+) (waaF) [Campylobacter jejuni strain NCTC12662]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDVSYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENATLVHLDLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLTNI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=219272 B2K12_RS05495 WP_002853794.1 1071215..1072165(+) (waaF) [Campylobacter jejuni strain NCTC12662]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCGCCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCTCAGTTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGCAAAATCGATTTTAGCTTTGCT
TTTAGGTCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAGCAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGTGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATTGCTTTTTGTGATCTTTTCATCACAAACGACAGT
GGACCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAACATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

93.61

99.051

0.927

Multiple sequence alignment