Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B2M23_RS08140 Genome accession   NZ_CP019962
Coordinates   1774360..1775721 (+) Length   453 a.a.
NCBI ID   WP_038352928.1    Uniprot ID   -
Organism   Eubacterium limosum strain ATCC 8486     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1769360..1780721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2M23_RS08115 (B2M23_08135) - 1769499..1770848 (+) 1350 WP_038352932.1 uroporphyrinogen decarboxylase family protein -
  B2M23_RS08120 (B2M23_08140) - 1770907..1771827 (+) 921 WP_038352931.1 CobW family GTP-binding protein -
  B2M23_RS08125 (B2M23_08145) - 1771837..1772478 (+) 642 WP_038352930.1 acetolactate decarboxylase -
  B2M23_RS08130 (B2M23_08150) - 1772746..1773795 (+) 1050 WP_052237325.1 PTS sugar transporter subunit IIC -
  B2M23_RS08135 (B2M23_08155) - 1773864..1774349 (+) 486 WP_038352929.1 VanZ family protein -
  B2M23_RS08140 (B2M23_08160) radA 1774360..1775721 (+) 1362 WP_038352928.1 DNA repair protein RadA Machinery gene
  B2M23_RS08150 (B2M23_08170) ruvA 1776126..1776737 (+) 612 WP_038352927.1 Holliday junction branch migration protein RuvA -
  B2M23_RS08155 (B2M23_08175) ruvB 1776749..1777777 (+) 1029 WP_038352926.1 Holliday junction branch migration DNA helicase RuvB -
  B2M23_RS08160 (B2M23_08180) queA 1777788..1778822 (+) 1035 WP_038352925.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  B2M23_RS08165 (B2M23_08185) tgt 1778835..1779944 (+) 1110 WP_038352924.1 tRNA guanosine(34) transglycosylase Tgt -
  B2M23_RS08170 (B2M23_08190) yajC 1779963..1780409 (+) 447 WP_038352923.1 preprotein translocase subunit YajC -
  B2M23_RS08175 (B2M23_08195) scfA 1780436..1780576 (+) 141 WP_013381915.1 six-cysteine ranthipeptide SCIFF -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50455.79 Da        Isoelectric Point: 8.3461

>NTDB_id=219242 B2M23_RS08140 WP_038352928.1 1774360..1775721(+) (radA) [Eubacterium limosum strain ATCC 8486]
MAKLKKKFVCQNCGYSTPKWMGRCTECGEWNSFVEELDMPTEQGKKNTLERAVYTKPKRIEEIIPQKEDRFKTKNKELDR
VLGGGIVPGGVILLGGDPGIGKSTILLQTTENLGSQGLKILYISGEESEQQLKMRAVRMKVKSQNIFFLSEINVPYIVDT
ILNEKPDLVIIDSIQTMYSPTITSAPGSVSQIRENANALMQIAKKDNISMILVGHVTKEGNIAGPRVLEHMVDTVLYFEG
EKYHTYRILRGVKNRFGSTNEIGIFEMAQDGLHEVANPSEMMLSSRPKNTCGSVVVPCMEGTRPLLIELQGLVSQTSFGN
PRRMATGMDYNRMVLLLAIMEKRLGLQLQNLDAYINVVGGMKIDEPALDLAVVSVLYSSFRNFEIPEDMMIIGEVGLTGE
VRNIQHIEKRLKEGAKLGFKRCIMPKGNARGLENTGLELLPVDNISKALNILK

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=219242 B2M23_RS08140 WP_038352928.1 1774360..1775721(+) (radA) [Eubacterium limosum strain ATCC 8486]
ATGGCAAAATTAAAAAAGAAATTTGTGTGCCAGAACTGCGGGTACAGCACGCCTAAATGGATGGGGCGCTGTACGGAATG
CGGCGAATGGAACTCCTTTGTGGAGGAGCTGGATATGCCCACCGAGCAGGGAAAAAAGAATACCCTTGAACGGGCTGTGT
ACACAAAACCCAAGCGTATTGAAGAGATCATTCCTCAGAAAGAGGATCGTTTTAAAACGAAGAATAAAGAGCTCGACCGT
GTCCTCGGCGGCGGCATTGTGCCCGGAGGCGTTATCCTTCTGGGCGGTGACCCGGGAATTGGAAAGTCGACCATTCTTCT
GCAGACGACGGAAAATCTTGGAAGCCAGGGGCTTAAAATCCTGTATATTTCAGGTGAGGAATCCGAGCAGCAGCTCAAAA
TGCGGGCGGTCCGGATGAAGGTGAAATCCCAGAATATTTTTTTCCTGTCTGAGATTAACGTCCCCTATATCGTGGATACT
ATTTTGAATGAAAAGCCTGATCTTGTCATCATTGACTCGATCCAGACAATGTACAGTCCCACAATCACCTCGGCTCCCGG
CAGCGTCAGTCAGATAAGGGAGAATGCCAACGCATTGATGCAGATTGCCAAAAAGGATAATATTTCAATGATTCTGGTCG
GGCATGTAACCAAGGAAGGCAATATTGCCGGCCCCCGCGTTTTGGAACACATGGTTGATACGGTTCTGTATTTTGAGGGT
GAAAAGTACCATACTTACCGGATACTCAGAGGAGTTAAAAACCGTTTTGGCTCAACCAACGAGATCGGCATTTTTGAAAT
GGCACAGGATGGTCTGCATGAGGTGGCAAATCCTTCTGAAATGATGCTGTCGAGCCGTCCGAAAAACACTTGTGGCTCTG
TGGTAGTGCCTTGTATGGAGGGGACACGTCCGCTTTTAATTGAGCTTCAGGGACTTGTATCTCAGACAAGCTTTGGCAAC
CCAAGGCGCATGGCCACCGGAATGGACTATAATCGTATGGTGCTTCTTTTGGCCATTATGGAAAAACGGCTGGGATTACA
GCTTCAGAATCTTGATGCATATATCAATGTGGTTGGGGGTATGAAAATAGATGAGCCGGCCCTTGACCTGGCCGTTGTGT
CTGTGCTATACTCAAGCTTCAGGAATTTTGAAATTCCAGAGGATATGATGATTATCGGCGAGGTTGGTCTGACCGGAGAG
GTCAGAAATATACAGCACATTGAAAAACGCCTTAAAGAAGGCGCAAAGCTTGGTTTTAAGCGCTGTATCATGCCAAAGGG
AAATGCCCGGGGCCTGGAGAATACAGGGCTTGAGCTGCTGCCGGTCGATAACATATCAAAGGCTTTAAATATATTGAAGT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(62-220)

Subunit ChlI of Mg-chelatase (ChlI)

(355-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

55.752

99.779

0.556
  radA Streptococcus mitis NCTC 12261

52.546

95.364

0.501
  radA Streptococcus pneumoniae Rx1

52.546

95.364

0.501
  radA Streptococcus pneumoniae D39

52.546

95.364

0.501
  radA Streptococcus pneumoniae R6

52.546

95.364

0.501
  radA Streptococcus pneumoniae TIGR4

52.546

95.364

0.501
  radA Streptococcus mitis SK321

52.546

95.364

0.501

Multiple sequence alignment