Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B1774_RS00270 Genome accession   NZ_CP019866
Coordinates   53765..55153 (+) Length   462 a.a.
NCBI ID   WP_077974899.1    Uniprot ID   A0AB33HUX7
Organism   Dehalococcoides mccartyi strain KBTCE3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 48765..60153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1774_RS00265 (B1774_00265) - 51071..53545 (+) 2475 WP_041340825.1 ATP-dependent Clp protease ATP-binding subunit -
  B1774_RS00270 (B1774_00270) radA 53765..55153 (+) 1389 WP_077974899.1 DNA repair protein RadA Machinery gene
  B1774_RS00275 (B1774_00275) - 55140..55823 (+) 684 WP_058292929.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  B1774_RS00280 (B1774_00280) ispF 55829..56302 (+) 474 WP_010935868.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  B1774_RS00285 (B1774_00285) cysS 56306..57682 (+) 1377 WP_058292931.1 cysteine--tRNA ligase -
  B1774_RS00295 (B1774_00295) - 57897..58895 (+) 999 WP_077974926.1 tyrosine-type recombinase/integrase -
  B1774_RS00300 (B1774_00300) - 58899..59126 (-) 228 WP_077974927.1 hypothetical protein -
  B1774_RS00305 (B1774_00305) - 59564..59800 (-) 237 WP_077974928.1 hypothetical protein -
  B1774_RS00310 (B1774_00310) - 59911..60099 (+) 189 WP_077974929.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 49490.91 Da        Isoelectric Point: 7.6049

>NTDB_id=218590 B1774_RS00270 WP_077974899.1 53765..55153(+) (radA) [Dehalococcoides mccartyi strain KBTCE3]
MEKSRRVYVCSNCGRESLKWLGRCPACQEWNTFEEKTVISPIGRKSEPARVISPAAELSSLQAAETTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDTGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFKRALVPAGANCHNVHNIQIIPVSTVKEAVKLALTGVKTETEDVFE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=218590 B1774_RS00270 WP_077974899.1 53765..55153(+) (radA) [Dehalococcoides mccartyi strain KBTCE3]
ATGGAAAAAAGCCGTAGAGTTTATGTTTGTTCTAACTGCGGGCGTGAGAGCCTGAAATGGCTGGGCCGCTGCCCCGCCTG
TCAGGAGTGGAATACTTTTGAAGAAAAAACGGTTATTTCACCCATAGGGCGTAAAAGTGAACCTGCCCGGGTTATCAGCC
CGGCGGCAGAGCTTTCCAGTTTGCAGGCAGCCGAAACCACCCGGCGGAGTTTATCCATTTCGGAGTTTAACCGGGTTTTA
GGCGGCGGCATAGTGCCGGGTTCGCTTATGCTTTTAGGGGGGGAACCGGGCATCGGCAAATCCACTCTGCTTCTTCAGGT
GGCGGCTTCGGTAGCCCAAAGCGGCGGCAAGGTAGTATATGTCTCCGGGGAAGAAAACCCCGCCCAGATAAAAATGCGCG
CCCAACGCCTGGGCATCAGCGGCGAAGGGCTTTTCCTTATGGCTGAGACTGACCTTAATGCCATTTTAGCCCAGCTTTCA
GTCCTCTGCCCGTCACTGGTCGTTATTGACTCTATCCAGACTGTATTTCTGCCGGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAATCAGCCCTCCGCCTGATGCAGTGGGCTAAAAGCAGCGGAGCCAGCGTATTTATAACCGCCCATG
TTACCAAAGAGGGAAACATAGCCGGGCCGCGTATACTGGAGCATATAGTAGATGTGGTTATGTACTTTGAGGGAGAGTCC
CAGAGTGCTTACCGTTTGGTGCGTTCGGTAAAAAACCGTTTCGGTTCTACCAACGAAGTGGGTATATTTGAAATGAAAAG
TGAGGGTCTGGTAGAAGTGGCCAACCCTTCCCAGATATTTTTATCAAACCGCCAGGCAAATACGGTCGGGTCGGCTGTAA
CCGCTGTGCTGGAGGGCAGCCGCCCGCTGCTGGTTGAGGTGCAGGCACTTACCAATACCACCAGTTTCGGCCAGCCCCGC
CGCACCGCCAACGGGGTGGATTTTAACCGCACCCTTATGATAGCCGCTGTCCTCTCCAAACGCCTGTCTATGCGTTTAGG
TACCCAGGACATAATAGTCAACGCTACCGGCGGTATCCGCCTGGATGAGCCGGCCGCAGATTTAGCTATAGCTCTGGCCA
TTGCTTCCAGTTACCGGGATACCGGCGTCTGCCCAGAAACCATTGCCTTGGGGGAGATTGGCCTTTCTGGTGAACTGCGG
ACTATTCCCCATCTGGAAAGGCGTCTTTCCGAGGCCAGCCGTCTGGGTTTTAAGAGGGCTTTAGTACCGGCCGGTGCTAA
CTGCCACAATGTACATAATATCCAGATAATTCCGGTTTCAACTGTCAAAGAGGCGGTGAAGCTGGCACTTACCGGGGTAA
AAACGGAGACCGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(342-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.109

99.351

0.498
  radA Streptococcus pneumoniae Rx1

48.359

98.918

0.478
  radA Streptococcus pneumoniae D39

48.359

98.918

0.478
  radA Streptococcus pneumoniae R6

48.359

98.918

0.478
  radA Streptococcus pneumoniae TIGR4

48.359

98.918

0.478
  radA Streptococcus mitis SK321

48.359

98.918

0.478
  radA Streptococcus mitis NCTC 12261

48.14

98.918

0.476

Multiple sequence alignment