Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   B1F85_RS26510 Genome accession   NZ_CP019732
Coordinates   5511685..5513280 (+) Length   531 a.a.
NCBI ID   WP_003381271.1    Uniprot ID   A0A2V0RCF3
Organism   Pseudomonas syringae pv. actinidiae strain CRAFRU 14.08     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5506685..5518280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1F85_RS26490 (B1F85_26765) pgeF 5508467..5509195 (-) 729 WP_017683543.1 peptidoglycan editing factor PgeF -
  B1F85_RS26495 (B1F85_26770) rluD 5509192..5510154 (-) 963 WP_003381266.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  B1F85_RS26500 (B1F85_26775) - 5510304..5511326 (+) 1023 WP_003381267.1 outer membrane protein assembly factor BamD -
  B1F85_RS26505 (B1F85_26780) - 5511456..5511695 (+) 240 WP_057403016.1 PP0621 family protein -
  B1F85_RS26510 (B1F85_26785) pilS 5511685..5513280 (+) 1596 WP_003381271.1 sensor histidine kinase Regulator
  B1F85_RS26515 (B1F85_26790) pilR 5513283..5514623 (+) 1341 WP_017683544.1 sigma-54-dependent transcriptional regulator Regulator
  B1F85_RS26520 (B1F85_26795) - 5515084..5515701 (+) 618 WP_003381275.1 TetR/AcrR family transcriptional regulator -
  B1F85_RS26525 (B1F85_26800) - 5515783..5516898 (+) 1116 WP_017683545.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 531 a.a.        Molecular weight: 58334.25 Da        Isoelectric Point: 6.7758

>NTDB_id=218188 B1F85_RS26510 WP_003381271.1 5511685..5513280(+) (pilS) [Pseudomonas syringae pv. actinidiae strain CRAFRU 14.08]
MTADTPSLTSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANQNLFRSGCWLYLVFNILVVVLLERPSRQAQLF
GLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARLQVSEGLAEQRAADVDSLEELNALILQRMRTGILVLDARRQVLLANQGALTLLGHERLVGQVID
AYSPQLVERLRQWLINPIMVPRSITTSSIGPVLQPSFAALNRGDQRQTLIFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEDLSAPDRRLGQIIHDQSQRIDRVIENVLQLSRRREAQPELLDLKVWLENFVSDFRSSAATD
QSIHVQISPGMLTTRMDAGQLTQIITNLVQNGLRYSSRSHPSAQVWLKLQPDRESDLPILEVLDDGPGVSEQHLSKIFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYLPRLHDGGSCLRITFAHPFKTS

Nucleotide


Download         Length: 1596 bp        

>NTDB_id=218188 B1F85_RS26510 WP_003381271.1 5511685..5513280(+) (pilS) [Pseudomonas syringae pv. actinidiae strain CRAFRU 14.08]
GTGACCGCTGATACGCCTTCGCTTACCAGCCCGCAGGCACAACGGATTCTGCGGCTGTATCACCTTTATCGACTGACCAT
TGGCGTCGTGCTGGTTCTGTTGATCTCCAGCAGTCTGGAAACCGAGTTGCTGCAACTGGCCAATCAGAATCTGTTCCGTT
CGGGTTGCTGGCTGTACCTAGTGTTCAACATTCTGGTGGTGGTGCTGCTGGAACGCCCCAGCCGCCAGGCGCAGCTGTTT
GGCTTGGCGATGACTGACGCAATCATGCTTTCCGCGCTGTTTTATGCGGCCGGCGGGCCGTCGAGCGGCATCGGCAACCT
GTTGATCGCGTCCGTGGCGATCAGCAACGTGCTGCTGCGAGGCCGTATTGGCCTGTTGATCGCCGCCGTTTCTGCCATTG
GCATTATCTACCTGACGTTCTATATCAGCTTCGGCACACCTTCGGTGTCCAACGACTACGTGCAGGCCGGCTCTCTGGGC
GCGCTGTGCTTCGCCGCCGCGCTGCTGGTGCAGGCACTGACTGCGCGCCTGCAAGTCAGCGAGGGGCTGGCCGAGCAAAG
GGCAGCGGATGTCGACAGCCTTGAAGAGCTCAACGCTCTGATCCTGCAACGCATGCGCACCGGCATTCTGGTGCTGGATG
CGCGCCGTCAGGTGTTGCTGGCCAATCAGGGGGCCTTGACGCTGCTGGGCCACGAACGACTGGTCGGCCAGGTCATCGAC
GCTTACTCGCCGCAGTTGGTAGAGCGCCTGCGCCAGTGGCTGATTAACCCGATCATGGTGCCGCGCAGCATCACCACTTC
ATCTATCGGGCCTGTGCTGCAACCCAGCTTTGCCGCGCTCAATCGGGGCGACCAGCGTCAAACGCTGATTTTCCTTGAGG
ACCTGTCGCAGATTGCCCATCAGGCGCAGCAGCTCAAGCTTGCCGCGCTGGGCCGCCTGACCGCAGGCATCGCCCACGAA
ATACGTAATCCGCTGGGCGCGGTCAGCCACGCTGCGCAGTTACTCAACGAGTCGGAAGACCTCAGCGCGCCGGACCGCCG
TCTGGGGCAGATTATTCATGATCAGTCGCAGCGCATTGATCGGGTGATTGAAAACGTCCTGCAACTGTCTCGCCGACGCG
AAGCACAGCCAGAACTGCTCGACCTGAAAGTCTGGCTGGAAAATTTTGTCAGCGACTTTCGCAGCTCGGCGGCTACGGAT
CAGTCGATTCATGTGCAAATTAGCCCCGGCATGCTGACCACTCGCATGGACGCCGGTCAACTGACCCAGATCATCACCAA
CCTGGTGCAGAACGGCTTACGTTACAGCAGCCGCAGTCACCCGTCGGCGCAAGTCTGGCTGAAGCTGCAGCCAGACCGGG
AAAGCGATTTACCGATTCTGGAAGTCCTGGACGACGGGCCGGGTGTCAGCGAACAACACCTGAGCAAGATTTTCGAACCG
TTTTTCACCACGGAAAGCAAAGGCACCGGCCTGGGTCTATACCTTTCCCGTGAATTGTGCGAAAGCAATCAGGCGCATCT
GGAATACCTCCCCCGACTACACGACGGTGGCAGCTGTTTGCGCATCACCTTCGCCCACCCGTTCAAAACGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-524)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V0RCF3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

61.654

100

0.618

Multiple sequence alignment