Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A8E96_RS14270 Genome accession   NZ_CP019670
Coordinates   1988685..1990190 (+) Length   501 a.a.
NCBI ID   WP_074802179.1    Uniprot ID   -
Organism   Burkholderia cenocepacia strain VC12802     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1983685..1995190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8E96_RS14245 (A8E96_14185) kdsD 1984916..1985899 (+) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -
  A8E96_RS14250 (A8E96_14190) - 1985899..1986435 (+) 537 WP_012329237.1 KdsC family phosphatase -
  A8E96_RS14255 (A8E96_14195) lptC 1986439..1987053 (+) 615 WP_054928051.1 LPS export ABC transporter periplasmic protein LptC -
  A8E96_RS14260 (A8E96_14200) lptA 1987074..1987739 (+) 666 WP_077218593.1 lipopolysaccharide transport periplasmic protein LptA -
  A8E96_RS14265 (A8E96_14205) lptB 1987736..1988512 (+) 777 WP_006477788.1 LPS export ABC transporter ATP-binding protein -
  A8E96_RS14270 (A8E96_14210) rpoN/rpoN1 1988685..1990190 (+) 1506 WP_074802179.1 RNA polymerase factor sigma-54 Machinery gene
  A8E96_RS14280 (A8E96_14215) hpf 1990349..1990708 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  A8E96_RS14285 (A8E96_14220) - 1990903..1991415 (+) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  A8E96_RS14290 (A8E96_14225) hprK 1991545..1992513 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  A8E96_RS14295 (A8E96_14230) rapZ 1992588..1993496 (+) 909 WP_006477783.1 RNase adapter RapZ -
  A8E96_RS14300 (A8E96_14235) - 1993524..1994159 (+) 636 WP_011546241.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54767.85 Da        Isoelectric Point: 4.8740

>NTDB_id=217714 A8E96_RS14270 WP_074802179.1 1988685..1990190(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain VC12802]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSTSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=217714 A8E96_RS14270 WP_074802179.1 1988685..1990190(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain VC12802]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCACCGCG
ACCAACGGCAGCAGCACGTCCAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGTTACGGGTC
CGGTGACGGCAACGACGGCCCACAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGATCATCTCTCGGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTACT
CGCCGACCTGCCGCCCGAACTGGAAATCGACGGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGTCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAACAGCA
GCTGCAGGAAGCGCGCTGGTTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment