Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A8E88_RS26870 Genome accession   NZ_CP019668
Coordinates   5752940..5754445 (+) Length   501 a.a.
NCBI ID   WP_077180265.1    Uniprot ID   -
Organism   Burkholderia cenocepacia strain VC7848     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5747940..5759445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8E88_RS26845 (A8E88_26785) kdsD 5749171..5750154 (+) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -
  A8E88_RS26850 (A8E88_26790) - 5750154..5750690 (+) 537 WP_011546236.1 KdsC family phosphatase -
  A8E88_RS26855 (A8E88_26795) lptC 5750694..5751308 (+) 615 WP_006477790.1 LPS export ABC transporter periplasmic protein LptC -
  A8E88_RS26860 (A8E88_26800) lptA 5751329..5751994 (+) 666 WP_012329239.1 lipopolysaccharide transport periplasmic protein LptA -
  A8E88_RS26865 (A8E88_26805) lptB 5751991..5752767 (+) 777 WP_006477788.1 LPS export ABC transporter ATP-binding protein -
  A8E88_RS26870 (A8E88_26810) rpoN/rpoN1 5752940..5754445 (+) 1506 WP_077180265.1 RNA polymerase factor sigma-54 Machinery gene
  A8E88_RS26880 (A8E88_26815) hpf 5754604..5754963 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  A8E88_RS26885 (A8E88_26820) - 5755158..5755670 (+) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  A8E88_RS26890 (A8E88_26825) hprK 5755800..5756768 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  A8E88_RS26895 (A8E88_26830) rapZ 5756843..5757751 (+) 909 WP_006477783.1 RNase adapter RapZ -
  A8E88_RS26900 (A8E88_26835) - 5757779..5758414 (+) 636 WP_011546241.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54755.86 Da        Isoelectric Point: 4.8740

>NTDB_id=217701 A8E88_RS26870 WP_077180265.1 5752940..5754445(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain VC7848]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSGTSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=217701 A8E88_RS26870 WP_077180265.1 5752940..5754445(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain VC7848]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCACCGCG
ACCAACGGCAGCGGCACGTCCAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
CGGTGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCACAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTACT
CGCCGACCTGCCGCCCGAACTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAACAGCA
GCTGCAGGAAGCGCGCTGGTTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment