Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CWI64_RS10025 Genome accession   NZ_CP025076
Coordinates   1863290..1865512 (+) Length   740 a.a.
NCBI ID   WP_001812033.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain BHN97x     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1858290..1870512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI64_RS10000 (CWI64_10000) - 1858607..1859797 (-) 1191 WP_000843708.1 ferric reductase-like transmembrane domain-containing protein -
  CWI64_RS10005 (CWI64_10005) - 1860092..1860751 (-) 660 WP_000443865.1 amino acid ABC transporter permease -
  CWI64_RS10010 (CWI64_10010) - 1860761..1861438 (-) 678 WP_000131134.1 amino acid ABC transporter permease -
  CWI64_RS10015 (CWI64_10015) - 1861451..1862215 (-) 765 WP_000624335.1 transporter substrate-binding domain-containing protein -
  CWI64_RS10020 (CWI64_10020) - 1862255..1863013 (-) 759 WP_001229594.1 amino acid ABC transporter ATP-binding protein -
  CWI64_RS10025 (CWI64_10025) recJ 1863290..1865512 (+) 2223 WP_001812033.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CWI64_RS10030 (CWI64_10030) - 1865687..1867348 (+) 1662 WP_000065637.1 ribonuclease J -
  CWI64_RS10035 (CWI64_10035) - 1867417..1868196 (+) 780 WP_000290013.1 alpha/beta hydrolase family protein -
  CWI64_RS10040 (CWI64_10040) murM 1868308..1869528 (+) 1221 WP_000286563.1 peptidoglycan bridge formation alanyltransferase MurM -

Sequence

Protein


Download         Length: 740 a.a.        Molecular weight: 82563.10 Da        Isoelectric Point: 4.3320

>NTDB_id=217352 CWI64_RS10025 WP_001812033.1 1863290..1865512(+) (recJ) [Streptococcus pneumoniae strain BHN97x]
MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEE
GENILVYGDYDADGMTSASIVKESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQVELLDLVAIGTIADMVSLTDENR
ILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEETVGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKN
EERKEIVQSIYEEAKTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAVDIFEALDP
YRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNLDEELDLEALSLETVKSFERLAPFGMDNQKP
IFYIKNFQVESARTMGAGNAHLKLKISKGEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVE
GVQLFNIRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKNDIDKAYYLTGYGTRDQ
FAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFEELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKD
QEMMALGTVQEIYDFLMEKE

Nucleotide


Download         Length: 2223 bp        

>NTDB_id=217352 CWI64_RS10025 WP_001812033.1 1863290..1865512(+) (recJ) [Streptococcus pneumoniae strain BHN97x]
TTGATAACACCTACTTATGAATGGCAGTTTGCCCTGCAGGTAGAAGATGCGGATTTTACAAAGATAGCCAAGAAGGCTGG
ACTGGGTCCTGAGGTGGCTCGGTTATTGTTTGAGAGAGGGATTCAGGACCAAGAAAGTCTGAAGAAGTTTTTAGAACCTT
CCTTGGAGGACTTACATGATGCTTATCTGCTCCATGATATGGACAAGGCAGTGGAGCGGATTCGTCAGGCTATTGAAGAA
GGGGAAAATATTCTCGTTTATGGAGACTATGATGCGGATGGCATGACTTCGGCTTCTATTGTGAAGGAAAGTTTGGAACA
ACTTGGTGCGGAGTGCCGAGTTTACCTGCCAAATCGTTTTACCGATGGCTATGGCCCTAATGCTAGTGTTTATAAATACT
TTATCGAGCAAGAAGGAATTTCCTTGATTGTGACGGTGGACAATGGGGTTGCTGGTCATGAGGCTATTGCATTGGCTCAG
TCTATGGGAGTAGATGTCATTGTGACAGACCATCATTCCATACCTGAAACCCTGCCAGATGCTTATGCTATTGTCCATCC
TGAACATCCAGATGCGGATTATCCTTTTAAATATTTGGCTGGTTGTGGAGTTGCTTTCAAGTTGGCTTGTGCCCTGTTAG
AAGAAGTGCAAGTGGAATTGCTTGATTTGGTCGCTATTGGAACTATTGCAGATATGGTGAGTCTGACGGATGAAAATCGT
ATCTTAGTTCAATATGGTCTGGAAATGTTGGGTCATACCCAGCGCATTGGTCTGCAAGAAATGCTGGACATGGCTGGGAT
TGCTGCTAACGAAGTAACAGAAGAAACGGTTGGTTTCCAGATTGCTCCTCGTTTGAATGCCTTGGGTCGCTTGGATGATC
CCAATCCTGCCATTGATTTGTTGACTGGATTTGATGATGAGGAAGCGCATGAGATTGCCCTTATGATTCACCAGAAAAAC
GAAGAGCGCAAGGAAATCGTTCAGTCTATCTATGAAGAAGCCAAGACCATGGTGGATCCTGAGAAGAAGGTTCAGGTCTT
GGCCAAGGAAGGCTGGAATCCTGGGGTTCTAGGAATCGTGGCTGGTCGTTTATTGGAAGAATTGGGACAGACAGTCATTG
TTCTTAATATAGAAGACGGTCGTGCCAAGGGCAGTGCTCGTAGTGTGGAAGCGGTCGATATTTTTGAAGCTCTGGATCCC
TATCGAGACCTCTTCATCGCCTTTGGAGGTCATGCAGGTGCAGCGGGTATGACGCTGGAAGTTGAGCAACTCTCAGATTT
ATCTCAGATTTTGGAAGATTATGTTCGTGAAAAAGGTGCAGATGCTGGTGGCAAGAATAAGTTAAACCTAGATGAAGAGT
TGGATTTGGAGGCACTTAGCTTGGAAACGGTCAAAAGTTTTGAACGTTTAGCTCCTTTTGGAATGGATAATCAGAAACCT
ATTTTTTATATCAAGAATTTTCAGGTCGAAAGTGCTCGTACTATGGGGGCAGGTAATGCCCATCTAAAGCTGAAAATTTC
CAAGGGTGAGGTGAGTTTTGAAGTGGTAGCCTTTGGTCAAGGCAGATGGGCGACAGAGTTTTCTCAAACCAAGAATCTAG
AGTTAGCGGTTAAATTGTCTGTCAACCAATGGAACGGCCAAACTGCCCTCCAGTTGATGATGATGGATGCGCGAGTGGAA
GGTGTTCAACTTTTTAACATTCGTGGAAAAAATGCAGTCTTGCCAGAAGGTGTTCCAGTCTTGGATTTTCCTGGAGAACT
GCCAAATCTTGCGGCTAGTGAAGCTGTTGTCGTAAAAAACATTCCAGAGGATATTACTCAGCTGAAGACCATTTTTCAGG
AACAGCATTTCTCTGCTGTCTATTTCAAAAATGATATTGACAAGGCCTATTATCTGACAGGTTATGGGACTAGAGATCAG
TTTGCCAAATTGTACAAGACTATTTACCAGTTCCCAGAGTTTGATATTCGCTACAAGCTGAAAGATTTGGCTGCATATCT
TAATATTCAACAAATCTTGCTGGTCAAGATGATTCAAGTATTTGAAGAACTAGGCTTTGTGACGATAAAAGATGGTGTGA
TGACAGTCAATAAAGAGGCGCCAAAGCGGGAGATAGGAGAAAGTCAAATTTACCAAAATCTCAAACAAACCGTTAAAGAC
CAAGAAATGATGGCGCTGGGTACGGTGCAAGAAATTTATGATTTTTTGATGGAAAAAGAGTAG

Domains

Predicted by InterProScan.

DHH family (DHH)

(83-228)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(593-735)

RecJ OB domain (RecJ_OB)

(451-556)

DHHA1 domain (DHHA1)

(317-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

99.324

100

0.993
  recJ Bacillus subtilis subsp. subtilis str. 168

36.917

100

0.385