Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CWI33_RS18275 Genome accession   NZ_CP025048
Coordinates   3608556..3608996 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SC516     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3603556..3613996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI33_RS18255 (CWI33_18250) coaE 3604046..3604666 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  CWI33_RS24920 - 3604691..3604735 (+) 45 WP_120795372.1 protein YacM -
  CWI33_RS18260 (CWI33_18255) guaC 3604891..3605934 (+) 1044 WP_001217338.1 GMP reductase -
  CWI33_RS18265 (CWI33_18260) hofC 3605969..3607171 (-) 1203 Protein_3367 protein transport protein HofC -
  CWI33_RS18270 (CWI33_18265) pilB 3607161..3608546 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  CWI33_RS18275 (CWI33_18270) pilA 3608556..3608996 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  CWI33_RS18285 (CWI33_18280) nadC 3609199..3610092 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWI33_RS18290 (CWI33_18285) ampD 3610180..3610731 (+) 552 WP_078182515.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CWI33_RS18295 (CWI33_18290) ampE 3610728..3611582 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  CWI33_RS18300 (CWI33_18295) aroP 3611625..3612998 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=216738 CWI33_RS18275 WP_000360904.1 3608556..3608996(-) (pilA) [Escherichia coli strain SC516]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=216738 CWI33_RS18275 WP_000360904.1 3608556..3608996(-) (pilA) [Escherichia coli strain SC516]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment