Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMA_RS04910 Genome accession   NC_003116
Coordinates   944903..946033 (+) Length   376 a.a.
NCBI ID   WP_002246907.1    Uniprot ID   -
Organism   Neisseria meningitidis Z2491     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 939903..951033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMA_RS04895 (NMA0976) lepB 940934..941953 (-) 1020 WP_002236844.1 signal peptidase I -
  NMA_RS04900 (NMA0977) lepA 942096..943889 (-) 1794 WP_002236845.1 translation elongation factor 4 -
  NMA_RS04905 (NMA0978) - 944034..944735 (-) 702 WP_002246090.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NMA_RS04910 (NMA0979) pilU 944903..946033 (+) 1131 WP_002246907.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMA_RS04915 (NMA0980) - 946067..947044 (+) 978 WP_002246898.1 DNA polymerase III subunit delta' -
  NMA_RS04920 (NMA0981) - 947048..947398 (+) 351 WP_002246878.1 PilZ domain-containing protein -
  NMA_RS04925 (NMA0982) - 947403..948182 (+) 780 WP_002246871.1 TatD family hydrolase -
  NMA_RS04930 - 948231..948437 (+) 207 WP_002217599.1 DUF333 domain-containing protein -
  NMA_RS04935 (NMA0984) grxD 948538..948849 (+) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  NMA_RS04945 (NMA0985) upp 948955..949581 (+) 627 WP_002246895.1 uracil phosphoribosyltransferase -
  NMA_RS04950 (NMA0986) - 949604..949924 (+) 321 WP_002214001.1 DUF2322 family protein -
  NMA_RS04955 (NMA0987) - 950175..950597 (+) 423 WP_002226495.1 protein Gly1ORF1 -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42157.28 Da        Isoelectric Point: 6.2885

>NTDB_id=21642 NMA_RS04910 WP_002246907.1 944903..946033(+) (pilU) [Neisseria meningitidis Z2491]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRQAQSSGPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=21642 NMA_RS04910 WP_002246907.1 944903..946033(+) (pilU) [Neisseria meningitidis Z2491]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTCGTGACGACCCATTTCCCGCCCGCAATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGTCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGTGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCACCGGATGTGATTCTGATCGGCG
AAATCCGCGACCGCGAAACAATGGACTACGCCATTGCTTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCATTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAATATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACTTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCAGGCGCAAAGTTCCGGTCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.943

93.617

0.561
  pilU Acinetobacter baylyi ADP1

57.726

91.223

0.527
  pilU Vibrio cholerae strain A1552

54.039

95.479

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.768

87.234

0.364

Multiple sequence alignment